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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SUCLG1 Polyclonal antibody (14923-1-AP)

    WB analysis using 14923-1-AP (same clone as 14923-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14923-1-AP (SUCLG1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14923-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat liver tissue using SUCLG1 Polyclonal antibody (14923-1-AP)

    WB analysis of rat liver using 14923-1-AP (same clone as 14923-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 14923-1-AP (SUCLG1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14923-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse kidney tissue using SUCLG1 Polyclonal antibody (14923-1-AP)

    IP experiment of mouse kidney using 14923-1-AP (same clone as 14923-1-PBS)

    IP result of anti-SUCLG1 (IP:14923-1-AP, 4ug; Detection:14923-1-AP 1:500) with mouse kidney tissue lysate 2800 ug. This data was developed using the same antibody clone with 14923-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SUCLG1 Polyclonal antibody (14923-1-AP)

    IHC staining of human liver cancer using 14923-1-AP (same clone as 14923-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 14923-1-AP (SUCLG1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14923-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using SUCLG1 Polyclonal antibody (14923-1-AP)

    IHC staining of mouse kidney using 14923-1-AP (same clone as 14923-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14923-1-AP (SUCLG1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14923-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using SUCLG1 Polyclonal antibody (14923-1-AP)

    IHC staining of mouse kidney using 14923-1-AP (same clone as 14923-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14923-1-AP (SUCLG1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14923-1-PBS in a different storage buffer formulation.

SUCLG1 Polyclonal antibody, PBS Only

SUCLG1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 14923-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14923-1-PBS targets SUCLG1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag6481

Product name: Recombinant human SUCLG1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-333 aa of BC000504

Sequence: MVSGSSGLAAARLLSRSFLLPQNGIRHCSYTASRQHLYVDKNTKIICQGFTGKQGTFHSQQALEYGTKLVGGTTPGKGGQTHLGLPVFNTVKEAKEQTGATASVIYVPPPFAAAAINEAIEAEIPLVVCITEGIPQQDMVRVKHKLLRQEKTRLIGPNCPGVINPGECKIGIMPGHIHKKGRIGIVSRSGTLTYEAVHQTTQVGLGQSLCVGIGGDPFNGTDFIDCLEIFLNDSATEGIILIGEIGGNAEENAAEFLKQHNSGPNSKPVVSFIAGLTAPPGRRMGHAGAIIAGGKGGAKEKISALQSAGVVVSMSPAQLGTTIYKEFEKRKML

相同性解析による交差性が予測される生物種
Full Name succinate-CoA ligase, alpha subunit
Calculated molecular weight 35 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC000504
Gene Symbol SUCLG1
Gene ID (NCBI) 8802
RRIDAB_2197177
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP53597
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SUCLG1(succinyl-CoA ligase [ADP/GDP-forming] subunit alpha, mitochondrial) is also named as SCS-alpha and belongs to the succinate/malate CoA ligase alpha subunit family. The SUCLG1 gene encodes a 35 kDa protein with a 40 amino acids transit peptide. This enzyme catalyzes the ATP- or GTP-dependent ligation of succinate and CoA to form succinyl-CoA. Defects in SUCLG1 are the cause of mitochondrial DNA depletion syndrome type 9 (MTDPS9).

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