Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, PC-3 cells, HuH-7 cells |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | BxPC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14231-1-AP targets STXBP4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5471 Product name: Recombinant human STXBP4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-245 aa of BC041485 Sequence: MIGVSFEEAKSIITGAKLSTRLESAWEIAFIRQKSDNIQPENLSCTSLIEASGEYGPQASTLSLFSSPPEILIPKTSSTPKTNNDILSSCEIKTGYNKTVQIPITSENSTVGLSNTDVASAWTENYGLQEKISLNPSVRFKAEKLEMALNYLGIQPTKEQHQALRQQVQADSKGTVSFGDFVQVARNLFCLQLDEVNVGAHEISNILDSQLLPCDSSEADEMERLKCERDDALKEVNTLKWPGQN 相同性解析による交差性が予測される生物種 |
| Full Name | syntaxin binding protein 4 |
| Calculated molecular weight | 62 kDa |
| Observed molecular weight | 62 kDa |
| GenBank accession number | BC041485 |
| Gene Symbol | STXBP4 |
| Gene ID (NCBI) | 252983 |
| RRID | AB_2196980 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6ZWJ1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
STXBP4, also known as Synip, plays a role in the translocation of transport vesicles from the cytoplasm to the plasma membrane. STXBP4 inhibits the translocation of GLUT4 from intracellular vesicles to the plasma membrane by STX4A binding and preventing the interaction between STX4A and VAMP2(PMID: 10394363).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for STXBP4 antibody 14231-1-AP | Download protocol |
| IHC protocol for STXBP4 antibody 14231-1-AP | Download protocol |
| WB protocol for STXBP4 antibody 14231-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |