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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using STXBP3 Polyclonal antibody (13764-1-AP)

    WB analysis of HepG2 using 13764-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 13764-1-AP (STXBP3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using STXBP3 Polyclonal antibody (13764-1-AP)

    WB analysis of K-562 using 13764-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 13764-1-AP (STXBP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of K-562 cells using STXBP3 Polyclonal antibody (13764-1-AP)

    IP experiment of K-562 using 13764-1-AP

    IP result of anti-STXBP3 (IP:13764-1-AP, 4ug; Detection:13764-1-AP 1:500) with K-562 cells lysate 1200ug.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using STXBP3 Polyclonal antibody (13764-1-AP)

    IHC staining of human lymphoma using 13764-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 13764-1-AP (STXBP3 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using STXBP3 Polyclonal antibody (13764-1-AP)

    IHC staining of human lymphoma using 13764-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 13764-1-AP (STXBP3 antibody) at dilution of 1:100 (under 40x lens).

STXBP3 Polyclonal antibody

STXBP3 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 13764-1-AP
Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

MUNC18 3, MUNC18C, Platelet Sec1 protein, Protein unc 18 homolog 3, Protein unc 18 homolog C

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, K-562 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

13764-1-AP targets STXBP3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag4747

Product name: Recombinant human STXBP3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 242-592 aa of BC038099

Sequence: FDPVSTVLHELTFQAMAYDLLPIENDTYKYKTDGKEKEAILEEEDDLWVRIRHRHIAVVLEEIPKLMKEISSTKKATEGKTSLSALTQLMKKMPHFRKQITKQVVHLNLAEDCMNKFKLNIEKLCKTEQDLALGTDAEGQKVKDSMRVLLPVLLNKNHDNCDKIRAILLYIFSINGTTEENLDRLIQNVKIENESDMIRNWSYLGVPIVPQSQQGKPLRKDRSAEETFQLSRWTPFIKDIMEDAIDNRLDSKEWPYCSQCPAVWNGSGAVSARQKPRANYLEDRKNGSKLIVFVIGGITYSEVRCAYEVSQAHKSCEVIIGSTHVLTPKKLLDDIKMLNKPKDKVSLIKDE

相同性解析による交差性が予測される生物種
Full Name syntaxin binding protein 3
Calculated molecular weight 592 aa, 68 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC038099
Gene Symbol STXBP3
Gene ID (NCBI) 6814
RRIDAB_2196832
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO00186
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for STXBP3 antibody 13764-1-APDownload protocol
IP protocol for STXBP3 antibody 13764-1-APDownload protocol
WB protocol for STXBP3 antibody 13764-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Diabetes

PMID: 20460426

Authors - Pontus Boström
View Article
mouseWB

J Biol Chem

microRNA-15b contributes to depression-like behavior in mice by affecting synaptic protein levels and function in the nucleus accumbens.

Authors - Li Guo
View Article
ratWB

Neuroscience

SNAP25/syntaxin4/VAMP2/Munc18-1 Complexes in Spinal Dorsal Horn Contributed to Inflammatory Pain.

Authors - Xing-Lian Duan
View Article
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