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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using STXBP1 Monoclonal antibody (67137-1-Ig)

    WB analysis using 67137-1-Ig (same clone as 67137-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67137-1-Ig (STXBP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67137-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Y79 cells using STXBP1 Monoclonal antibody (67137-1-Ig)

    WB analysis of Y79 using 67137-1-Ig (same clone as 67137-1-PBS)

    Y79 cells were subjected to SDS PAGE followed by western blot with 67137-1-Ig (STXBP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67137-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using STXBP1 Monoclonal antibody (67137-1-Ig)

    IHC staining of mouse brain using 67137-1-Ig (same clone as 67137-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67137-1-Ig (STXBP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67137-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using STXBP1 Monoclonal antibody (67137-1-Ig)

    IHC staining of mouse brain using 67137-1-Ig (same clone as 67137-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67137-1-Ig (STXBP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67137-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using STXBP1 Monoclonal antibody (67137-1-Ig)

    IHC staining of mouse cerebellum using 67137-1-Ig (same clone as 67137-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67137-1-Ig (STXBP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67137-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using STXBP1 Monoclonal antibody (67137-1-Ig)

    IHC staining of mouse cerebellum using 67137-1-Ig (same clone as 67137-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67137-1-Ig (STXBP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67137-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using STXBP1 Monoclonal antibody (67137-1-Ig)

    IHC staining of mouse cerebellum using 67137-1-Ig (same clone as 67137-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67137-1-Ig (STXBP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67137-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using STXBP1 Monoclonal antibody (67137-1-Ig)

    IF Staining of mouse brain using 67137-1-Ig (same clone as 67137-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using STXBP1 antibody (67137-1-Ig, Clone: 1B5B3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67137-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using STXBP1 Monoclonal antibody (67137-1-Ig)

    IF Staining of mouse brain using 67137-1-Ig (same clone as 67137-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using STXBP1 antibody (67137-1-Ig, Clone: 1B5B3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67137-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using STXBP1 Monoclonal antibody (67137-1-Ig)

    IF Staining of mouse brain using 67137-1-Ig (same clone as 67137-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using STXBP1 antibody (67137-1-Ig, Clone: 1B5B3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67137-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using STXBP1 Monoclonal antibody (67137-1-Ig)

    FC experiment of HeLa using 67137-1-Ig (same clone as 67137-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human STXBP1 (67137-1-Ig, Clone:1B5B3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 67137-1-PBS in a different storage buffer formulation.

STXBP1 Monoclonal antibody, PBS Only

STXBP1 Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), Indirect ELISA
Cat No. 67137-1-PBS
Clone No.1B5B3

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, FC (Intra), Indirect ELISA

Synonyms

1B5B3, EIEE4, FLJ37475, MUNC18 1, Munc18-1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67137-1-PBS targets STXBP1 in WB, IHC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag28605

Product name: Recombinant human STXBP1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 245-594 aa of BC015749

Sequence: HELTFQAMSYDLLPIENDVYKYETSGIGEARVKEVLLDEDDDLWIALRHKHIAEVSQEVTRSLKDFSSSKRMNTGEKTTMRDLSQMLKKMPQYQKELSKYSTHLHLAEDCMKHYQGTVDKLCRVEQDLAMGTDAEGEKIKDPMRAIVPILLDANVSTYDKIRIILLYIFLKNGITEENLNKLIQHAQIPPEDSEIITNMAHLGVPIVTDSTLRRRSKPERKERISEQTYQLSRWTPIIKDIMEDTIEDKLDTKHYPYISTRSSASFSTTAVSARYGHWHKNKAPGEYRSGPRLIIFILGGVSLNEMRCAYEVTQANGKWEVLIGSTHILTPQKLLDTLKKLNKTDEEISS

相同性解析による交差性が予測される生物種
Full Name syntaxin binding protein 1
Calculated molecular weight 594 aa, 68 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC015749
Gene Symbol STXBP1
Gene ID (NCBI) 6812
RRIDAB_2882436
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP61764
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

STXBP1, also named as UNC18A, N-Sec1 and p67 belongs to the STXBP/unc-18/SEC1 family. STXBP1 may participate in the regulation of synaptic vesicle docking and fusion, possibly through interaction with GTP-binding proteins. It is essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1:1 ratio. STXBP1 can interact with syntaxins 1, 2, and 3 but not syntaxin 4. It may play a role in determining the specificity of intracellular fusion reactions.

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