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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Syntaxin 6 Recombinant antibody (84627-5-RR)

    WB analysis using 84627-5-RR (same clone as 84627-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84627-5-RR (Syntaxin 6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84627-5-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Syntaxin 6 Recombinant antibody (84627-5-RR)

    WB analysis using 84627-5-RR (same clone as 84627-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84627-5-RR (Syntaxin 6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84627-5-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using Syntaxin 6 Recombinant antibody (84627-5-RR)

    IP experiment of mouse brain using 84627-5-RR (same clone as 84627-5-PBS)

    IP result of anti-Syntaxin 6 (IP:84627-5-RR, 4ug; Detection:84627-5-RR 1:1500) with mouse brain tissue lysate 1840 ug. This data was developed using the same antibody clone with 84627-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Syntaxin 6 Recombinant antibody (84627-5-RR)

    IHC staining of mouse brain using 84627-5-RR (same clone as 84627-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84627-5-RR (Syntaxin 6 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84627-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of PC-12 cells using Syntaxin 6 Recombinant antibody (84627-5-RR)

    IF Staining of PC-12 using 84627-5-RR (same clone as 84627-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using Syntaxin 6 antibody (84627-5-RR, Clone: 242100A9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84627-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84627-5-RR

    Affinity and Kinetic Characterization of 84627-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84627-5-RR against Human Syntaxin 6 were performed. The affinity constant is 1.55 nM.

Syntaxin 6 Recombinant monoclonal antibody, PBS Only

Syntaxin 6 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 84627-5-PBS
Clone No.242100A9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Syntaxin-6, STX6, 242100A9

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84627-5-PBS targets Syntaxin 6 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag1284

Product name: Recombinant human STX6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-255 aa of BC009944

Sequence: MSMEDPFFVVKGEVQKAVNTAQGLFQRWTELLQDPSTATREEIDWTTNELRNNLRSIEWDLEDLDETISIVEANPRKFNLDATELSIRKAFITSTRQVVRDMKDQMSTSSVQALAERKNRQALLGDSGSQNWSTGTTDKYGRLDRELQRANSHFIEEQQAQQQLIVEQQDEQLELVSGSIGVLKNMSQRIGGELEEQAVMLEDFSHELESTQSRLDNVMKKLAKVSHMTSDRRQWCAIAILFAVLLVVLILFLVL

相同性解析による交差性が予測される生物種
Full Name syntaxin 6
Calculated molecular weight 29 kDa
Observed molecular weight32 kDa
GenBank accession numberBC009944
Gene Symbol Syntaxin 6
Gene ID (NCBI) 10228
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDO43752
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Syntaxin-6 (STX6) is a SNARE protein located in the trans-Golgi network (TGN) and endosomes. It associates with a variety of SNARE proteins and is involved in intracellular vesicle trafficking. STX6 is required for sorting proteins from endosomes toward either the TGN or lysosomes.