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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using STX3 Monoclonal antibody (66760-1-Ig)

    WB analysis using 66760-1-Ig (same clone as 66760-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66760-1-Ig (STX3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66760-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using STX3 Monoclonal antibody (66760-1-Ig)

    WB analysis using 66760-1-Ig (same clone as 66760-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66760-1-Ig (STX3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66760-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using STX3 Monoclonal antibody (66760-1-Ig)

    WB analysis of HEK-293 using 66760-1-Ig (same clone as 66760-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66760-1-Ig (STX3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66760-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SH-SY5Y cells using STX3 Monoclonal antibody (66760-1-Ig)

    WB analysis of SH-SY5Y using 66760-1-Ig (same clone as 66760-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 66760-1-Ig (STX3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66760-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using STX3 Monoclonal antibody (66760-1-Ig)

    WB analysis of rat brain using 66760-1-Ig (same clone as 66760-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 66760-1-Ig (STX3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66760-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using STX3 Monoclonal antibody (66760-1-Ig)

    IHC staining of human kidney using 66760-1-Ig (same clone as 66760-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66760-1-Ig (STX3 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66760-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using STX3 Monoclonal antibody (66760-1-Ig)

    IHC staining of human kidney using 66760-1-Ig (same clone as 66760-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66760-1-Ig (STX3 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66760-1-PBS in a different storage buffer formulation.

STX3 Monoclonal antibody, PBS Only

STX3 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66760-1-PBS
Clone No.1B9C7

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

STX3, STX3A, syntaxin 3

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66760-1-PBS targets STX3 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Reactivity Human, rat, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag7805

Product name: Recombinant human STX3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-264 aa of BC007405

Sequence: MKDRLEQLKAKQLTQDDDTDAVEIAIDNTAFMDEFFSEIEETRLNIDKISEHVEEAKKLYSIILSAPIPEPKTKDDLEQLTTEIKKRANNVRNKLKSMEKHIEEDEVRSSADLRIRKSQHSVLSRKFVEVMTKYNEAQVDFRERSKGRIQRQLEITGKKTTDEELEEMLESGNPAIFTSGIIDSQISKQALSEIEGRHKDIVRLESSIKELHDMFMDIAMLVENQGEMLDNIELNVMHTVDHVEKARDETKKAVKYQSQARKKL

相同性解析による交差性が予測される生物種
Full Name syntaxin 3
Calculated molecular weight 33 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC007405
Gene Symbol STX3
Gene ID (NCBI) 6809
RRIDAB_2882106
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ13277
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Syntaxin-3 (also known as STX3) is a member of the syntaxin. Syntaxin-3 is a significant apical targeting protein in the epithelial membrane and in exocytosis process; it also acts as a vesicle transporter by cellular receptor in neutrophils, which is crucial for protein trafficking event (PMID: 25630102). It has been reported that syntaxin-3 has an important role in the growth of neurites, and serves as a direct target for omega-6 arachidonic acid (PMID: 1659826).

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