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Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using STX1A Polyclonal antibody (55164-1-AP)

    WB analysis of human plasma using 55164-1-AP

    human plasma was subjected to SDS PAGE followed by western blot with 55164-1-AP (STX1A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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STX1A Polyclonal antibody

STX1A Polyclonal Antibody for WB, ELISA
Cat No. 55164-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

syntaxin 1A (brain), Syntaxin 1A, STX1, p35 1, Neuron specific antigen HPC 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inhuman plasma

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55164-1-AP targets STX1A in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name syntaxin 1A (brain)
Calculated molecular weight 33 kDa
Observed molecular weight35 kDa
GenBank accession numberNM_004603
Gene Symbol STX1A
Gene ID (NCBI) 6804
RRIDAB_3086433
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16623
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Syntaxins are a family of transmembrane proteins that belong to the SNARE complex (PMID: 11737951). In conjunction with other SNAREs and with the cytoplasmic NSF and SNAP proteins, syntaxins mediate vesicle fusion in diverse vesicular transport processes along the exocytic and endocytic pathways (PMID: 11737951). Syntaxin 1A (STX1A) and 1B (STX1B), two closely related isoforms of syntaxin 1, are involved in synaptic vesicle docking and fusion during neurotransmitter release (PMID: 7690687; 1321498; 18691641). Syntaxin 1A is associated with a variety of neurological diseases, including epilepsy, attention deficit hyperactivity disorder (ADHD), migraine, etc (PMID: 26918652; 25445064; 22250207). This antibody recognizes human STX1A.

Protocols

Product Specific Protocols
WB protocol for STX1A antibody 55164-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols