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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Syntaxin 12 Recombinant antibody (85069-4-RR)

    WB analysis using 85069-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85069-4-RR (Syntaxin 12 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using Syntaxin 12 Recombinant antibody (85069-4-RR)

    IF Staining of A431 using 85069-4-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using Syntaxin 12 antibody (85069-4-RR, Clone: 242546H2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 85069-4-RR

    Affinity and Kinetic Characterization of 85069-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85069-4-RR against Human Syntaxin 12 were performed. The affinity constant is 1.05 nM.

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Syntaxin 12 Recombinant antibody

Syntaxin 12 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85069-4-RR
Clone No.242546H2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Syntaxin-12, STX12, 242546H2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inLNCaP cells, HepG2 cells, HEK-293 cells, Jurkat cells, human placenta tissue
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85069-4-RR targets Syntaxin 12 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Syntaxin 12 fusion protein Ag5532 相同性解析による交差性が予測される生物種
Full Name syntaxin 12
Calculated molecular weight 32 kDa
Observed molecular weight39 kDa
GenBank accession numberBC046999
Gene Symbol Syntaxin 12
Gene ID (NCBI) 23673
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ86Y82
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Syntaxin 12 (STX12), also known as STX13 or STX14, is a 276 amino acid single-pass type IV membrane protein that belongs to the syntaxin family. It is a broadly and differentially expressed SNARE protein, highly enriched in brain tissue. STX12 is localized to early and recycling endosomes and is involved in the recycling of plasma membrane proteins. It also has a role in the trafficking of MMP during the degradation of ECM substrates and subsequent cellular invasion. (PMID: 9507000; 9553086; 19910495) This antibody recognizes a major 39 kDa band of endogenous STX12. In some cases, two additional bands of 33 kDa and 66 kDa could also be detected, which most likely represent a degradation product and a SNARE complex respectively (PMID: 9817754).

Protocols

Product Specific Protocols
WB protocol for Syntaxin 12 antibody 85069-4-RRDownload protocol
IF protocol for Syntaxin 12 antibody 85069-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols