Validation Data Gallery
Tested Applications
| Positive WB detected in | rat brain tissue, human brain tissue, mouse liver tissue, rat liver tissue |
| Positive IHC detected in | human liver tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 6 publications below |
| WB | See 26 publications below |
| IHC | See 2 publications below |
| IF | See 2 publications below |
| IP | See 2 publications below |
| CoIP | See 2 publications below |
Product Information
15323-1-AP targets STT3B in WB, IHC, IF/ICC, IP, CoIP, ELISA, Blocking assay applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2039 Product name: Recombinant human STT3B protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-225 aa of BC015880 Sequence: MSWWDYGYQIAGMANRTTLVDNNTWNNSHIALVGKAMSSNETAAYKIMRTLDVDYVLVIFGGVIGYSGDDINKFLWMVRIAEGEHPKDIRESDYFTPQGEFRVDKAGSPTLLNCLMYKMSYYRFGEMQLDFRTPPGFDRTRNAEIGNKDIKFKHLEEAFTSEHWLVRIYKVKAPDNRETLDHKPRVTNIFPKQKYLSKKTTKRKRGYIKNKLVFKKGKKIFKKTV 相同性解析による交差性が予測される生物種 |
| Full Name | STT3, subunit of the oligosaccharyltransferase complex, homolog B (S. cerevisiae) |
| Calculated molecular weight | 97 kDa |
| Observed molecular weight | 94 kDa |
| GenBank accession number | BC015880 |
| Gene Symbol | STT3B |
| Gene ID (NCBI) | 201595 |
| RRID | AB_2198046 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8TCJ2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
STT3B is a component of the N-oligosaccharyl transferase (OST) enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). SST3B seems to be involved in complex substrate specificity
Publications
| Species | Application | Title |
|---|---|---|
Nature Structural basis of regulated N-glycosylation at the secretory translocon.
| ||
Int J Oral Sci Stabilization of EREG via STT3B-mediated N-glycosylation is critical for PDL1 upregulation and immune evasion in head and neck squamous cell carcinoma | ||
Nat Chem Biol Oligosaccharyltransferase inhibition induces senescence in RTK-driven tumor cells. | ||
J Clin Invest Integrated screening identifies GPR31 as a key driver and druggable target for metabolic dysfunction-associated steatohepatitis | ||
Adv Sci (Weinh) N-glycosylation Modification of CTSD Affects Liver Metastases in Colorectal Cancer
| ||
Sci Adv Signal recognition particle receptor-β (SR-β) coordinates cotranslational N-glycosylation |