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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using STRN Recombinant antibody (85058-4-RR)

    WB analysis using 85058-4-RR (same clone as 85058-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85058-4-RR (STRN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85058-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using STRN Recombinant antibody (85058-4-RR)

    IHC staining of mouse brain using 85058-4-RR (same clone as 85058-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85058-4-RR (STRN antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85058-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using STRN Recombinant antibody (85058-4-RR)

    IHC staining of mouse brain using 85058-4-RR (same clone as 85058-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85058-4-RR (STRN antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85058-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using STRN Recombinant antibody (85058-4-RR)

    IF Staining of mouse brain using 85058-4-RR (same clone as 85058-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using STRN antibody (85058-4-RR, Clone: 242532D8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85058-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using STRN Recombinant antibody (85058-4-RR)

    IF Staining of mouse brain using 85058-4-RR (same clone as 85058-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using STRN antibody (85058-4-RR, Clone: 242532D8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85058-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85058-4-RR

    Affinity and Kinetic Characterization of 85058-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85058-4-RR against Human STRN were performed. The affinity constant is 0.433 nM.

STRN Recombinant monoclonal antibody, PBS Only

STRN Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 85058-4-PBS
Clone No.242532D8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

242532D8, SG2NA, Striatin

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85058-4-PBS targets STRN in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag15984

Product name: Recombinant human STRN protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 301-403 aa of BC106879

Sequence: NRSKLQDMLANLRDVDELPSLQPSVGSPSRPSSSRLPEHEINRADEVEALTFPPSSGKSFIMGADEALESELGLGELAGLTVANEADSLTYDIANNKDALRKT

相同性解析による交差性が予測される生物種
Full Name striatin, calmodulin binding protein
Calculated molecular weight 780 aa, 86 kDa
Observed molecular weight 110 kDa
GenBank accession numberBC106879
Gene Symbol STRN
Gene ID (NCBI) 6801
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDO43815
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

STRN (Striatin), a 780-amino acid protein, was identified and cloned more than a decade ago. In adult mammals, striatin is mainly expressed in neurons of both the central and peripheral nervous systems with very high levels of expression in the striatum, hence its name. STRN-ALK fusion may be a potential therapeutic target in the aggressive forms of thyroid cancer (PMID: 24475247).

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