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Validation Data Gallery

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  • Western Blot (WB) analysis of Raji cells using STRADB Polyclonal antibody (10688-1-AP)

    WB analysis of Raji using 10688-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 10688-1-AP (STRADB Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of mouse heart tissue using STRADB Polyclonal antibody (10688-1-AP)

    WB analysis of mouse heart using 10688-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 10688-1-AP (STRADB Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

  • Immunohistochemistry (IHC) staining of human heart tissue using STRADB Polyclonal antibody (10688-1-AP)

    IHC staining of human heart using 10688-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10688-1-AP (STRADB Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using STRADB Polyclonal antibody (10688-1-AP)

    IHC staining of human heart using 10688-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10688-1-AP (STRADB Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using STRADB Polyclonal antibody (10688-1-AP)

    IHC staining of mouse liver using 10688-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10688-1-AP (STRADB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human rectal cancer tissue using STRADB Polyclonal antibody (10688-1-AP)

    IHC staining of human rectal cancer using 10688-1-AP

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 10688-1-AP (STRADB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using STRADB Polyclonal antibody (10688-1-AP)

    IHC staining of mouse kidney using 10688-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10688-1-AP (STRADB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using STRADB Polyclonal antibody (10688-1-AP)

    IHC staining of mouse pancreas using 10688-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 10688-1-AP (STRADB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat kidney tissue using STRADB Polyclonal antibody (10688-1-AP)

    IHC staining of rat kidney using 10688-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 10688-1-AP (STRADB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat liver tissue using STRADB Polyclonal antibody (10688-1-AP)

    IHC staining of rat liver using 10688-1-AP

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 10688-1-AP (STRADB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

STRADB Polyclonal antibody

STRADB Polyclonal Antibody for WB, IHC, ELISA
Cat No. 10688-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

ALS2CR2, Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 2 protein, CALS 21, ILP interacting protein, ILP-interacting protein

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inRaji cells, mouse heart tissue
Positive IHC detected inhuman heart tissue, mouse liver tissue, mouse kidney tissue, rat kidney tissue, rat liver tissue, human rectal cancer tissue, mouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10688-1-AP targets STRADB in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1040

Product name: Recombinant human STRADB protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 119-418 aa of BC008302

Sequence: ITTYWTVFTVGSWLWVISPFMAYGSASQLLRTYFPEGMSETLIRNILFGAVRGLNYLHQNGCIHRSIKASHILISGDGLVTLSGLSHLHSLVKHGQRHRAVYDFPQFSTSVQPWLSPELLRQDLHGYNVKSDIYSVGITACELASGQVPFQDMHRTQMLLQKLKGPPYSPLDISIFPQSESRMKNSQSGVDSGIGESVLVSSGTHTVNSDRLHTPSSKTFSPAFFSLVQLCLQQDPEKRPSASSLLSHVFFKQMKEESQDSILSLLPPAYNKPSISLPPVLPWTEPECDFPDEKDSYWEF

相同性解析による交差性が予測される生物種
Full Name STE20-related kinase adaptor beta
Calculated molecular weight 47 kDa
Observed molecular weight 28-31 kDa, 38-42 kDa
GenBank accession numberBC008302
Gene Symbol STRADB
Gene ID (NCBI) 55437
RRIDAB_2197715
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9C0K7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The 418 amino acid STRADB protein, which is also named as ALS2CR2 and ILPIP, contains a putative N-terminal ATP-binding site, a protein kinase domain, and several C-terminal phosphorylation sites. STRADB potentiates the antiapoptotic activity of XIAP by enhancing XIAP-mediated activation of JNK1 and other JNK family members, but not by modulating XIAP-mediated caspase inhibition. This protein has 3 isoforms produced by alternative splicing with the MW of 47 kDa, 42 kDa and 31 kDa.

Protocols

Product Specific Protocols
IHC protocol for STRADB antibody 10688-1-APDownload protocol
WB protocol for STRADB antibody 10688-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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