Validation Data Gallery
Tested Applications
| Positive WB detected in | Raji cells, mouse heart tissue |
| Positive IHC detected in | human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10688-1-AP targets STRADB in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1040 Product name: Recombinant human STRADB protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 119-418 aa of BC008302 Sequence: ITTYWTVFTVGSWLWVISPFMAYGSASQLLRTYFPEGMSETLIRNILFGAVRGLNYLHQNGCIHRSIKASHILISGDGLVTLSGLSHLHSLVKHGQRHRAVYDFPQFSTSVQPWLSPELLRQDLHGYNVKSDIYSVGITACELASGQVPFQDMHRTQMLLQKLKGPPYSPLDISIFPQSESRMKNSQSGVDSGIGESVLVSSGTHTVNSDRLHTPSSKTFSPAFFSLVQLCLQQDPEKRPSASSLLSHVFFKQMKEESQDSILSLLPPAYNKPSISLPPVLPWTEPECDFPDEKDSYWEF 相同性解析による交差性が予測される生物種 |
| Full Name | STE20-related kinase adaptor beta |
| Calculated molecular weight | 47 kDa |
| Observed molecular weight | 28-31 kDa, 38-42 kDa |
| GenBank accession number | BC008302 |
| Gene Symbol | STRADB |
| Gene ID (NCBI) | 55437 |
| RRID | AB_2197715 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9C0K7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The 418 amino acid STRADB protein, which is also named as ALS2CR2 and ILPIP, contains a putative N-terminal ATP-binding site, a protein kinase domain, and several C-terminal phosphorylation sites. STRADB potentiates the antiapoptotic activity of XIAP by enhancing XIAP-mediated activation of JNK1 and other JNK family members, but not by modulating XIAP-mediated caspase inhibition. This protein has 3 isoforms produced by alternative splicing with the MW of 47 kDa, 42 kDa and 31 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for STRADB antibody 10688-1-AP | Download protocol |
| WB protocol for STRADB antibody 10688-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |