Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, A549 cells, human liver tissue, human lung tissue, K-562 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IHC | See 3 publications below |
| CoIP | See 1 publications below |
Product Information
12046-1-AP targets Stomatin in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2676 Product name: Recombinant human STOM protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-288 aa of BC010703 Sequence: MAEKRHTRDSEAQRLPDSFKDSPSKGLGPCGWILVAFSFLFTVITFPISIWMCIKIIKEYERAIIFRLGRILQGGAKGPGLFFILPCTDSFIKVDMRTISFDIPPQEILTKDSVTISVDGVVYYRVQNATLAVANITNADSATRLLAQTTLRNVLGTKNLSQILSDREEIAHNMQSTLDDATDAWGIKVERVEIKDVKLPVQLQRAMAAEAEASREARAKVIAAEGEMNASRALKEASMVITEYPAALQLRYLQTLTTIAAEKNSTIVFPLPIDMLQGIIGAKHSHLG 相同性解析による交差性が予測される生物種 |
| Full Name | stomatin |
| Calculated molecular weight | 288 aa, 32 kDa |
| Observed molecular weight | 32 kDa |
| GenBank accession number | BC010703 |
| Gene Symbol | Stomatin |
| Gene ID (NCBI) | 2040 |
| RRID | AB_2197406 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P27105 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
STOM, also named as BND7, EPB72, Stomatin and Protein 7.2b, belongs to the band 7/mec-2 family. It is thought to regulate cation conductance. STOM may regulate ACCN1 and ACCN3 gating. STOM is a membrane protein involved in regulation of monovalent cation transport through lipid membranes. Interestingly, STOM (which has a structure similar to caveolin) is a major lipid-raft component of erythrocytes. It may have a role in membrane microdomains modulation leading to membrane budding and MPs release.(PMID:19138258)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Stomatin antibody 12046-1-AP | Download protocol |
| IHC protocol for Stomatin antibody 12046-1-AP | Download protocol |
| IP protocol for Stomatin antibody 12046-1-AP | Download protocol |
| WB protocol for Stomatin antibody 12046-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
BMC Cancer Role of arachidonic acid metabolism in osteosarcoma prognosis by integrating WGCNA and bioinformatics analysis | ||
Front Med Facile discovery of red blood cell deformation and compromised membrane/skeleton assembly in Prader-Willi syndrome | ||
Front Oncol Upregulation of stomatin is associated with poor prognosis and promotes tumor progression of orbital diffuse large B-cell lymphoma | ||
Vox Sang Microparticles in stored red blood cells: an approach using flow cytometry and proteomic tools. | ||
Chin J Cancer Res Stomatin plays a suppressor role in non-small cell lung cancer metastasis. | ||
Biophys J Interaction with stomatin directs human proton channels into cholesterol-dependent membrane domains |
