Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of HeLa using 12046-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 12046-1-AP (Stomatin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 12046-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 12046-1-AP (STOM antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human lung using 12046-1-AP
human lung tissue were subjected to SDS PAGE followed by western blot with 12046-1-AP (STOM antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 12046-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 12046-1-AP (STOM antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 12046-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 12046-1-AP (Stomatin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 12046-1-AP
IP result of anti-Stomatin (IP:12046-1-AP, 3ug; Detection:12046-1-AP 1:300) with HeLa cells lysate 2800ug.
IHC staining of human lung cancer using 12046-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12046-1-AP (Stomatin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 12046-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12046-1-AP (Stomatin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of A549 using 12046-1-AP
Immunofluorescent analysis of (4% PFA) fixed A549 cells using Stomatin antibody (12046-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Stomatin Polyclonal antibody

Stomatin Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 12046-1-AP

Publications(8)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

STOM, BND7, EPB7, EPB72, Erythrocyte membrane protein band 7.2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (8)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, A549 cells, human liver tissue, human lung tissue, K-562 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human lung cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A549 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 4 publications below
IHC	See 3 publications below
CoIP	See 1 publications below

Product Information

12046-1-AP targets Stomatin in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2676 Product name: Recombinant human STOM protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-288 aa of BC010703 Sequence: MAEKRHTRDSEAQRLPDSFKDSPSKGLGPCGWILVAFSFLFTVITFPISIWMCIKIIKEYERAIIFRLGRILQGGAKGPGLFFILPCTDSFIKVDMRTISFDIPPQEILTKDSVTISVDGVVYYRVQNATLAVANITNADSATRLLAQTTLRNVLGTKNLSQILSDREEIAHNMQSTLDDATDAWGIKVERVEIKDVKLPVQLQRAMAAEAEASREARAKVIAAEGEMNASRALKEASMVITEYPAALQLRYLQTLTTIAAEKNSTIVFPLPIDMLQGIIGAKHSHLG 相同性解析による交差性が予測される生物種
Full Name	stomatin
Calculated molecular weight	288 aa, 32 kDa
Observed molecular weight	32 kDa
GenBank accession number	BC010703
Gene Symbol	Stomatin
Gene ID (NCBI)	2040
RRID	AB_2197406
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P27105
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

STOM, also named as BND7, EPB72, Stomatin and Protein 7.2b, belongs to the band 7/mec-2 family. It is thought to regulate cation conductance. STOM may regulate ACCN1 and ACCN3 gating. STOM is a membrane protein involved in regulation of monovalent cation transport through lipid membranes. Interestingly, STOM (which has a structure similar to caveolin) is a major lipid-raft component of erythrocytes. It may have a role in membrane microdomains modulation leading to membrane budding and MPs release.(PMID:19138258)

Protocols

Product Specific Protocols
IF protocol for Stomatin antibody 12046-1-AP	Download protocol
IHC protocol for Stomatin antibody 12046-1-AP	Download protocol
IP protocol for Stomatin antibody 12046-1-AP	Download protocol
WB protocol for Stomatin antibody 12046-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IHC	Chin J Cancer Res Stomatin plays a suppressor role in non-small cell lung cancer metastasis. Authors - Huaying An View Article
human	IHC	BMC Cancer Role of arachidonic acid metabolism in osteosarcoma prognosis by integrating WGCNA and bioinformatics analysis Authors - Yaling Wang View Article
		J Biol Chem Association of protein arginine deiminase 4 with the myosin-9 motor complex Authors - Xiaoxing Wang View Article
human	WB,CoIP	Biophys J Interaction with stomatin directs human proton channels into cholesterol-dependent membrane domains Authors - Artem G Ayuyan View Article
human	WB	Front Med Facile discovery of red blood cell deformation and compromised membrane/skeleton assembly in Prader-Willi syndrome Authors - Yashuang Yang View Article
human	WB,IHC	Front Oncol Upregulation of stomatin is associated with poor prognosis and promotes tumor progression of orbital diffuse large B-cell lymphoma Authors - Wen Chen View Article

Validation Data Gallery

WB analysis of HeLa using 12046-1-AP

WB analysis of human liver using 12046-1-AP

WB analysis of human lung using 12046-1-AP

WB analysis of K-562 using 12046-1-AP

WB analysis of A549 using 12046-1-AP

IP experiment of HeLa using 12046-1-AP

IHC staining of human lung cancer using 12046-1-AP

IHC staining of human lung cancer using 12046-1-AP

IF Staining of A549 using 12046-1-AP

Stomatin Polyclonal antibody

Stomatin Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Stomatin plays a suppressor role in non-small cell lung cancer metastasis.

Role of arachidonic acid metabolism in osteosarcoma prognosis by integrating WGCNA and bioinformatics analysis

Association of protein arginine deiminase 4 with the myosin-9 motor complex

Interaction with stomatin directs human proton channels into cholesterol-dependent membrane domains

Facile discovery of red blood cell deformation and compromised membrane/skeleton assembly in Prader-Willi syndrome

Upregulation of stomatin is associated with poor prognosis and promotes tumor progression of orbital diffuse large B-cell lymphoma

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