Validation Data Gallery
Tested Applications
| Positive WB detected in | SH-SY5Y cells, Neuro-2a cells, PC-12 cells, mouse brain tissue, rat brain tissue |
| Positive IP detected in | Neuro-2a cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
RMX00021 targets STMN2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Multi-Recombinant |
| Type | Antibody |
| Immunogen |
Recombinant protein 相同性解析による交差性が予測される生物種 |
| Full Name | stathmin-like 2 |
| Calculated molecular weight | 21 kDa |
| Observed molecular weight | 18-21 kDa |
| GenBank accession number | BC006302 |
| Gene Symbol | STMN2 |
| Gene ID (NCBI) | 11075 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | N/A |
| UNIPROT ID | Q93045 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Stathmin-2 (encoded by STMN2), a member of the stathmin family of proteins, encodes a phosphoprotein that has an important role in microtubule dynamics. The STMN2 protein promotes microtubule dynamics in axonal growth cones , and is thought to play an important role in neurite outgrowth. STMN2 protein is localized in both Golgi and cytosolic compartments. Its expression appears modulated in osteoblasts by nerve growth factor, dexamethasone or RhoA kinase inhibitor Y-27632 which are known effectors of osteogenesis. (PMID: 33841129, PMID: 18611392)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for STMN2 antibody RMX00021 | Download protocol |
| IHC protocol for STMN2 antibody RMX00021 | Download protocol |
| IP protocol for STMN2 antibody RMX00021 | Download protocol |
| WB protocol for STMN2 antibody RMX00021 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |