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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

STMN2 Recombinant antibody

STMN2 Recombinant Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

230110B12

Cat no : 82925-2-RR

Synonyms

Protein SCG10, SCG10, SCGN10, SGC10, Stathmin 2, stathmin like 2, STMN2

Validation Data Gallery

Applications:
  • WB analysis of SH-SY5Y using 82925-2-RR

    WB analysis of SH-SY5Y using 82925-2-RR

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 82925-2-RR (STMN2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IP experiment of SH-SY5Y using 82925-2-RR

    IP experiment of SH-SY5Y using 82925-2-RR

    IP result of anti-STMN2 (IP:82925-2-RR, 4ug; Detection:82925-2-RR 1:1500) with SH-SY5Y cells lysate 1240 ug.

  • IHC staining of mouse brain using 82925-2-RR

    IHC staining of mouse brain using 82925-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82925-2-RR (STMN2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with STMN2 antibody included
  • IHC staining of mouse brain using 82925-2-RR

    IHC staining of mouse brain using 82925-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82925-2-RR (STMN2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with STMN2 antibody included
  • IF Staining of SH-SY5Y using 82925-2-RR

    IF Staining of SH-SY5Y using 82925-2-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using STMN2 antibody (82925-2-RR, Clone: 230110B12 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inSH-SY5Y cells
Positive IP detected inSH-SY5Y cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells
Planning an IHC experiment? We recommend our IHCeasy STMN2 Ready-To-Use IHC Kit. STMN2 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

82925-2-RR targets STMN2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen STMN2 fusion protein Ag0929 相同性解析による交差性が予測される生物種
Full Name stathmin-like 2
Calculated molecular weight 21 kDa
Observed molecular weight 18-21 kDa
GenBank accession numberBC006302
Gene symbol STMN2
Gene ID (NCBI) 11075
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Stathmin-2 (encoded by STMN2), a member of the stathmin family of proteins, encodes a phosphoprotein that has an important role in microtubule dynamics. The STMN2 protein promotes microtubule dynamics in axonal growth cones , and is thought to play an important role in neurite outgrowth. STMN2 protein is localized in both Golgi and cytosolic compartments. Its expression appears modulated in osteoblasts by nerve growth factor, dexamethasone or RhoA kinase inhibitor Y-27632 which are known effectors of osteogenesis. (PMID: 33841129, PMID: 18611392)

Protocols

Product Specific Protocols
WB protocol for STMN2 antibody 82925-2-RRDownload protocol
IHC protocol for STMN2 antibody 82925-2-RRDownload protocol
IF protocol for STMN2 antibody 82925-2-RRDownload protocol
IP protocol for STMN2 antibody 82925-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols