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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using STMN2 Recombinant monoclonal antibody (82925-1-RR)

    WB analysis using 82925-1-RR (same clone as 82925-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82925-1-RR (STMN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82925-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using STMN2 Recombinant monoclonal antibody (82925-1-RR)

    IP experiment of mouse brain using 82925-1-RR (same clone as 82925-1-PBS)

    IP result of anti-STMN2 (IP:82925-1-RR, 4ug; Detection:82925-1-RR 1:5000) with mouse brain tissue lysate 1800 ug. This data was developed using the same antibody clone with 82925-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using STMN2 Recombinant monoclonal antibody (82925-1-RR)

    IHC staining of mouse brain using 82925-1-RR (same clone as 82925-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82925-1-RR (STMN2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82925-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using STMN2 Recombinant monoclonal antibody (82925-1-RR)

    IHC staining of mouse brain using 82925-1-RR (same clone as 82925-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82925-1-RR (STMN2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82925-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using STMN2 Recombinant monoclonal antibody (82925-1-RR)

    IF Staining of mouse brain using 82925-1-RR (same clone as 82925-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using STMN2 antibody (82925-1-RR, Clone: 230110D5 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82925-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using STMN2 Recombinant monoclonal antibody (82925-1-RR)

    IF Staining of SH-SY5Y using 82925-1-RR (same clone as 82925-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using STMN2 antibody (82925-1-RR, Clone: 230110D5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82925-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of SH-SY5Y cells using STMN2 Recombinant monoclonal antibody (82925-1-RR)

    FC experiment of SH-SY5Y using 82925-1-RR (same clone as 82925-1-PBS)

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug STMN2 Recombinant antibody (82925-1-RR, Clone:230110D5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 82925-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82925-1-RR

    Affinity and Kinetic Characterization of 82925-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82925-1-RR against Human STMN2 were performed. The affinity constant is 1.29 nM.

STMN2 Recombinant monoclonal antibody, PBS Only

STMN2 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA
Cat No. 82925-1-PBS
Clone No.230110D5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA

Synonyms

230110D5, SCG 10, SCG10, SCGN 10, SCGN10

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82925-1-PBS targets STMN2 in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag0929

Product name: Recombinant human STMN2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-179 aa of BC006302

Sequence: MAKTAMAYKEKMKELSMLSLICSCFYPEPRNINIYTYDDMEVKQINKRASGQAFELILKPPSPISEAPRTLASPKKKDLSLEEIQKKLEAAEERRKSQEAQVLKQLAEKREHEREVLQKALEENNNFSKMAEEKLILKMEQIKENREANLAAIIERLQEKERHAAEVRRNKELQVELSG

相同性解析による交差性が予測される生物種
Full Name stathmin-like 2
Calculated molecular weight 21 kDa
Observed molecular weight 18-21 kDa
GenBank accession numberBC006302
Gene Symbol STMN2
Gene ID (NCBI) 11075
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ93045
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Stathmin-2 (encoded by STMN2), a member of the stathmin family of proteins, encodes a phosphoprotein that has an important role in microtubule dynamics. The STMN2 protein promotes microtubule dynamics in axonal growth cones , and is thought to play an important role in neurite outgrowth. STMN2 protein is localized in both Golgi and cytosolic compartments. Its expression appears modulated in osteoblasts by nerve growth factor, dexamethasone or RhoA kinase inhibitor Y-27632 which are known effectors of osteogenesis. (PMID: 33841129, PMID: 18611392)

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