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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using STK39 Polyclonal antibody (31064-1-AP)

    WB analysis using 31064-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31064-1-AP (STK39 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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STK39 Polyclonal antibody

STK39 Polyclonal Antibody for WB, ELISA
Cat No. 31064-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

DCHT, PASK, SPAK, Ste 20 related kinase

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, SH-SY5Y cells, SK-N-SH cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31064-1-AP targets STK39 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen STK39 fusion protein Ag34503 相同性解析による交差性が予測される生物種
Full Name serine threonine kinase 39 (STE20/SPS1 homolog, yeast)
Calculated molecular weight59 kDa
Observed molecular weight68 kDa
GenBank accession numberBC166614
Gene Symbol STK39
Gene ID (NCBI) 27347
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ9UEW8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

STK39 (Serine/Threonine Kinase 39) is a proline- and alanine-rich Ste20-related kinase (also named as SPAK), which is composed of a short N-terminal proline and alanine repeats (PAPA box), a kinase catalytic domain and a C-terminal regulatory domain (PMID: 16382158). In mammalians, STK39 plays an important role in ion homeostasis by regulating the cation chloride cotransporters' activities, which is crucial for the modulation of renal salt transport and blood pressure (PMID: 33500714). Notably, STK39 is also implicated in the regulation of tumor cell proliferation, migration, and invasion in multiple cancers, including osteosarcoma and cervical cancer (PMID: 34335956).

Protocols

Product Specific Protocols
WB protocol for STK39 antibody 31064-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols