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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using STK38 Polyclonal antibody (55335-1-AP)

    WB analysis of MCF-7 using 55335-1-AP (same clone as 55335-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 55335-1-AP (STK38 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55335-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using STK38 Polyclonal antibody (55335-1-AP)

    WB analysis of MCF-7 using 55335-1-AP (same clone as 55335-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 55335-1-AP (STK38 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55335-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using STK38 Polyclonal antibody (55335-1-AP)

    WB analysis of HeLa using 55335-1-AP (same clone as 55335-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 55335-1-AP (STK38 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55335-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using STK38 Polyclonal antibody (55335-1-AP)

    IHC staining of human kidney using 55335-1-AP (same clone as 55335-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55335-1-AP (STK38 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 55335-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using STK38 Polyclonal antibody (55335-1-AP)

    IHC staining of human kidney using 55335-1-AP (same clone as 55335-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55335-1-AP (STK38 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 55335-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using STK38 Polyclonal antibody (55335-1-AP)

    IHC staining of human breast cancer using 55335-1-AP (same clone as 55335-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55335-1-AP (STK38 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55335-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using STK38 Polyclonal antibody (55335-1-AP)

    IHC staining of human breast cancer using 55335-1-AP (same clone as 55335-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55335-1-AP (STK38 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55335-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using STK38 Polyclonal antibody (55335-1-AP)

    IF Staining of HEK-293 using 55335-1-AP (same clone as 55335-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using STK38 antibody (55335-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 55335-1-PBS in a different storage buffer formulation.

STK38 Polyclonal antibody, PBS Only

STK38 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 55335-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Nuclear Dbf2-related kinase 1, Nuclear Dbf2 related kinase 1, NDR1 protein kinase, NDR1, NDR

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

55335-1-PBS targets STK38 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name serine/threonine kinase 38
Calculated molecular weight 54 kDa
Observed molecular weight 54 kDa
GenBank accession numberNM_007271
Gene Symbol STK38
Gene ID (NCBI) 11329
RRIDAB_2881310
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15208
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Serine/threonine-protein kinase 38 belongs to AGC Ser/Thr protein kinase family.STK38 implicates in cell proliferation and/or tumor progression, and might play a role in the HIV-1 life cycle.Researches showed that STK38 with STK38L, were proapoptotic kinases and key members of the RASSF1/STK4 signaling cascade and its kinase activity increased in early mitotic phase and was dependent on FRY and STK3.This antibody recognizes the C-terminal of STK38.

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