Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | A549 cells, HeLa cells, MCF-7 cells, T-47D cells, JAR cells |
| Positive IHC detected in | human colon cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67528-1-Ig targets STK32A in WB, IHC, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10942 Product name: Recombinant human STK32A protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-166 aa of BC021666 Sequence: MGANTSRKPPVFDENEDVNFDHFEILRAIGKGSFGKVCIVQKNDTKKMYAMKYMNKQKCVERNEVRNVFKELQIMQGLEHPFLVNLWYSFQDEEDMFMVVDLLLGGDLRYHLQQNVHFKEETVKLFICELVMALDYLQNQRIIHRDMKPDNILLDEHDTWLSYKSH 相同性解析による交差性が予測される生物種 |
| Full Name | serine/threonine kinase 32A |
| Calculated molecular weight | 396aa,46 kDa; 166aa,20 kDa |
| Observed molecular weight | 50 kDa |
| GenBank accession number | BC021666 |
| Gene Symbol | STK32A |
| Gene ID (NCBI) | 202374 |
| RRID | AB_2882747 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q8WU08 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for STK32A antibody 67528-1-Ig | Download protocol |
| WB protocol for STK32A antibody 67528-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
