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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

STK32A Monoclonal antibody

STK32A Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1H2A6

Cat no : 67528-1-Ig

Synonyms

serine/threonine kinase 32A, STK32A, YANK1

Validation Data Gallery

Applications:
  • WB analysis of A549 using 67528-1-Ig

    WB analysis of A549 using 67528-1-Ig

    A549 cells were subjected to SDS PAGE followed by western blot with 67528-1-Ig (STK32A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 67528-1-Ig

    WB analysis of HeLa using 67528-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 67528-1-Ig (STK32A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of MCF-7 using 67528-1-Ig

    WB analysis of MCF-7 using 67528-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 67528-1-Ig (STK32A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of T-47D using 67528-1-Ig

    WB analysis of T-47D using 67528-1-Ig

    T-47D cells were subjected to SDS PAGE followed by western blot with 67528-1-Ig (STK32A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of JAR using 67528-1-Ig

    WB analysis of JAR using 67528-1-Ig

    JAR cells were subjected to SDS PAGE followed by western blot with 67528-1-Ig (STK32A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 67528-1-Ig

    IHC staining of human colon cancer using 67528-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67528-1-Ig (STK32A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 67528-1-Ig

    IHC staining of human colon cancer using 67528-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67528-1-Ig (STK32A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 67528-1-Ig

    IHC staining of human breast cancer using 67528-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67528-1-Ig (STK32A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inA549 cells, HeLa cells, MCF-7 cells, T-47D cells, JAR cells
Positive IHC detected inhuman colon cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

67528-1-Ig targets STK32A in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen STK32A fusion protein Ag10942 相同性解析による交差性が予測される生物種
Full Name serine/threonine kinase 32A
Calculated molecular weight396aa,46 kDa; 166aa,20 kDa
Observed molecular weight50 kDa
GenBank accession numberBC021666
Gene symbol STK32A
Gene ID (NCBI) 202374
RRIDAB_2882747
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for STK32A antibody 67528-1-IgDownload protocol
IHC protocol for STK32A antibody 67528-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols