Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis using 12097-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12097-1-AP (STK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 12097-1-AP
WB result of STK3 antibody (12097-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-STK3 transfected HeLa cells.
IHC staining of human pancreas cancer using 12097-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12097-1-AP (STK3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 12097-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12097-1-AP (STK3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 12097-1-AP
Immunofluorescent analysis of Hela cells, using STK3 antibody 12097-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG (green). Blue pseudocolor = DAPI (fluorescent DNA dye).
IF Staining of HeLa using 12097-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 12097-1-AP (STK3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

STK3 Polyclonal antibody

STK3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 12097-1-AP

Publications(10)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

MST-2, MST2, MST 2, Mammalian STE20-like protein kinase 2, KRS1

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (10)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A375 cells, HeLa cells, HL-60 cells
Positive IHC detected in	human pancreas cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	Hela cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 10 publications below
IHC	See 3 publications below
IF	See 2 publications below

Product Information

12097-1-AP targets STK3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, rat, sheep
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2741 Product name: Recombinant human STK3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-306 aa of BC010640 Sequence: MEQPPAPKSKLKKLSEDSLTKQPEEVFDVLEKLGEGSYGSVFKAIHKESGQVVAIKQVPVESDLQEIIKEISIMQQCDSPYVVKYYGSYFKNTDLWIVMEYCGAGSVSDIIRLRNKTLIEDEIATILKSTLKGLEYLHFMRKIHRDIKAGNILLNTEGHAKLADFGVAGQLTDTMAKRNTVIGTPFWMAPEVIQEIGYNCVADIWSLGITSIEMAEGKPPYADIHPMRAIFMIPTNPPPTFRKPELWSDDFTDFVKKCLVKNPEQRATATQLLQHPFIKNAKPVSILRDLITEAMEIKAKRHEEQQ 相同性解析による交差性が予測される生物種
Full Name	serine/threonine kinase 3 (STE20 homolog, yeast)
Calculated molecular weight	491 aa, 56 kDa
Observed molecular weight	63 kDa
GenBank accession number	BC010640
Gene Symbol	STK3
Gene ID (NCBI)	6788
RRID	AB_2198801
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q13188
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for STK3 antibody 12097-1-AP	Download protocol
IHC protocol for STK3 antibody 12097-1-AP	Download protocol
IF protocol for STK3 antibody 12097-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	EMBO J MST2 methylation by PRMT5 inhibits Hippo signaling and promotes pancreatic cancer progression Authors - Yan Sun View Article
human	WB	Aging (Albany NY) LINC00968 can inhibit the progression of lung adenocarcinoma through the miR-21-5p/SMAD7 signal axis. Authors - Yuxing Zhu View Article
human	WB	Mol Cancer Ther Peptide PDHPS1 inhibits ovarian cancer growth through disrupting YAP signaling. Authors - Xinxing Pan View Article
human	WB	Cancer Sci The crosstalk between AXL and YAP promotes tumor progression through STAT3 activation in head and neck squamous cell carcinoma. Authors - Jiayi Li View Article
human	IF,IHC,WB	Cell Signal Effect of STK3 on proliferation and apoptosis of pancreatic cancer cells via PI3K/AKT/mTOR pathway Authors - Jun Chen KD Validated View Article
human	WB	Molecules Essential Oil from Pinus Koraiensis Pinecones Inhibits Gastric Cancer Cells via the HIPPO/YAP Signaling Pathway. Authors - Yandong Zhang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 12097-1-AP

WB analysis of HeLa using 12097-1-AP

IHC staining of human pancreas cancer using 12097-1-AP

IHC staining of human pancreas cancer using 12097-1-AP

IF Staining of HeLa using 12097-1-AP

IF Staining of HeLa using 12097-1-AP

STK3 Polyclonal antibody

STK3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

MST2 methylation by PRMT5 inhibits Hippo signaling and promotes pancreatic cancer progression

LINC00968 can inhibit the progression of lung adenocarcinoma through the miR-21-5p/SMAD7 signal axis.

Peptide PDHPS1 inhibits ovarian cancer growth through disrupting YAP signaling.

The crosstalk between AXL and YAP promotes tumor progression through STAT3 activation in head and neck squamous cell carcinoma.

Effect of STK3 on proliferation and apoptosis of pancreatic cancer cells via PI3K/AKT/mTOR pathway

Essential Oil from Pinus Koraiensis Pinecones Inhibits Gastric Cancer Cells via the HIPPO/YAP Signaling Pathway.