Validation Data Gallery
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Tested Applications
| Positive WB detected in | A375 cells, HeLa cells, HL-60 cells |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Hela cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 13 publications below |
| IHC | See 5 publications below |
| IF | See 2 publications below |
Product Information
12097-1-AP targets STK3/MST2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat, canine, sheep |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2741 Product name: Recombinant human STK3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-306 aa of BC010640 Sequence: MEQPPAPKSKLKKLSEDSLTKQPEEVFDVLEKLGEGSYGSVFKAIHKESGQVVAIKQVPVESDLQEIIKEISIMQQCDSPYVVKYYGSYFKNTDLWIVMEYCGAGSVSDIIRLRNKTLIEDEIATILKSTLKGLEYLHFMRKIHRDIKAGNILLNTEGHAKLADFGVAGQLTDTMAKRNTVIGTPFWMAPEVIQEIGYNCVADIWSLGITSIEMAEGKPPYADIHPMRAIFMIPTNPPPTFRKPELWSDDFTDFVKKCLVKNPEQRATATQLLQHPFIKNAKPVSILRDLITEAMEIKAKRHEEQQ 相同性解析による交差性が予測される生物種 |
| Full Name | serine/threonine kinase 3 (STE20 homolog, yeast) |
| Calculated molecular weight | 491 aa, 56 kDa |
| Observed molecular weight | 56-63 kDa |
| GenBank accession number | BC010640 |
| Gene Symbol | STK3 |
| Gene ID (NCBI) | 6788 |
| RRID | AB_2198801 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13188 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for STK3/MST2 antibody 12097-1-AP | Download protocol |
| IHC protocol for STK3/MST2 antibody 12097-1-AP | Download protocol |
| WB protocol for STK3/MST2 antibody 12097-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) TEAD1 Enhances Exosome Secretion and Promotes Exosome-Mediated Tissue Regeneration. | ||
Cell Death Dis Mitoguardin 1 and 2 promote granulosa cell proliferation by activating AKT and regulating the Hippo-YAP1 signaling pathway | ||
J Immunother Cancer Overcoming immunotherapy resistance in bladder cancer with a novel antibody-drug conjugate RC48 | ||
EMBO J MST2 methylation by PRMT5 inhibits Hippo signaling and promotes pancreatic cancer progression | ||
Cell Mol Gastroenterol Hepatol RASSF4 attenuates metabolic dysfunction-associated steatotic liver disease progression via Hippo signaling and suppresses hepatocarcinogenesis | ||
Mol Cancer Ther Peptide PDHPS1 inhibits ovarian cancer growth through disrupting YAP signaling. |