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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using STK25 Recombinant antibody (86216-1-RR)

    WB analysis using 86216-1-RR (same clone as 86216-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86216-1-RR (STK25 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86216-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP02398-1

    Sandwich ELISA standard curve of MP02398-1

    Sandwich ELISA standard curve of MP02398-1, Human STK25 Recombinant Matched Antibody Pair - PBS only. 86216-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag22964. 86216-1-PBS was HRP conjugated as the detection antibody. Range: 62.5-4000 pg/mL

  • Sample test of MP02398-1

    Sample test of MP02398-1

    The mean SNAI2/SLUG concentration was determined to be 4,108.0 pg/mL in LnCap cell extract based on a 2.0 mg/mL extract load and 3,191.3 pg/mL in U2OS cell extract based on a 2.2 mg/mL extract load.

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STK25 Recombinant antibody, PBS Only (Detector)

STK25 Uni-rAbTM Recombinant Antibody for WB, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 86216-1-PBS
Clone No.250202A6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

EC:2.7.11.1, Serine/threonine-protein kinase 25, SOK 1, SOK1, SOK-1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86216-1-PBS targets STK25 as part of a matched antibody pair:

MP02398-1: 86216-2-PBS capture and 86216-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen STK25 fusion protein Ag22964 相同性解析による交差性が予測される生物種
Full Name serine/threonine kinase 25 (STE20 homolog, yeast)
Calculated molecular weight 48 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC015793
Gene Symbol STK25
Gene ID (NCBI) 10494
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO00506
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.