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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using STK11/LKB1 Polyclonal antibody (29323-1-AP)

    WB analysis using 29323-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29323-1-AP (STK11 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using STK11/LKB1 Polyclonal antibody (29323-1-AP)

    WB analysis of MCF-7 using 29323-1-AP

    WB result of STK11/LKB1 antibody (29323-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STK11/LKB1 transfected MCF-7 cells.

  • Western Blot (WB) analysis of A431 cells using STK11/LKB1 Polyclonal antibody (29323-1-AP)

    WB analysis of A431 using 29323-1-AP

    WB result of STK11/LKB1 antibody (29323-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STK11/LKB1 transfected A431 cells.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using STK11/LKB1 Polyclonal antibody (29323-1-AP)

    IHC staining of human lung cancer using 29323-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29323-1-AP (STK11/LKB1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using STK11/LKB1 Polyclonal antibody (29323-1-AP)

    IHC staining of human breast cancer using 29323-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 29323-1-AP (STK11/LKB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using STK11/LKB1 Polyclonal antibody (29323-1-AP)

    IHC staining of human breast cancer using 29323-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 29323-1-AP (STK11/LKB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using STK11/LKB1 Polyclonal antibody (29323-1-AP)

    IHC staining of mouse testis using 29323-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29323-1-AP (STK11/LKB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using STK11/LKB1 Polyclonal antibody (29323-1-AP)

    IHC staining of mouse testis using 29323-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29323-1-AP (STK11/LKB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using STK11/LKB1 Polyclonal antibody (29323-1-AP)

    IHC staining of human lymphoma using 29323-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 29323-1-AP (STK11/LKB1 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using STK11/LKB1 Polyclonal antibody (29323-1-AP)

    IHC staining of human lymphoma using 29323-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 29323-1-AP (STK11/LKB1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using STK11/LKB1 Polyclonal antibody (29323-1-AP)

    IHC staining of human lung cancer using 29323-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29323-1-AP (STK11/LKB1 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO Validated

STK11/LKB1 Polyclonal antibody

STK11/LKB1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29323-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse and More (1)

Applications

WB, IHC, ELISA

Synonyms

LKB1, PJS, serine/threonine kinase 11, STK11

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA431 cells, MCF-7 cells, HEK-293T cells
Positive IHC detected inhuman lung cancer tissue, human breast cancer tissue, mouse testis tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

29323-1-AP targets STK11/LKB1 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag30903

Product name: Recombinant human STK11 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 281-428 aa of BC007981

Sequence: PLSDLLKGMLEYEPAKRFSIRQIRQHSWFRKKHPPAEAPVPIPPSPDTKDRWRSMTVVPYLEDLHGADEDEDLFDIEDDIIYTQDFTVPGQVPEEEASHNGQRRGLPKAVCMNGTEAAQLSTKSRAEGRAPNPARKACSASSKIRRLS

相同性解析による交差性が予測される生物種
Full Name serine/threonine kinase 11
Calculated molecular weight 49 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC007981
Gene Symbol LKB1
Gene ID (NCBI) 6794
RRIDAB_2923591
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15831
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

STK11(serine/threonine-protein kinase 11) is also named as LKB1, PJS, and belongs to the protein kinase superfamily. It controls the activity of AMP-activated protein kinase (AMPK) family members, thereby playing a role in various processes such as cell metabolism, cell polarity, apoptosis and DNA damage response. The tumour suppressor protein LKB1 is a serine/threonine kinase that has been causally linked to Peutz-Jeghers syndrome (PJS). Defects in STK11 are a cause of Peutz-Jeghers syndrome (PJS) and defects in STK11 have been associated with testicular germ cell tumor (TGCT) and some sporadic cancers, especially lung cancers. STK11 has 2 isoforms with MW of 49 kDa and 45 kDa, and can be detected as 50-54 kDa after posttranslational modification.

Protocols

Product Specific Protocols
IHC protocol for STK11/LKB1 antibody 29323-1-APDownload protocol
WB protocol for STK11/LKB1 antibody 29323-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Free Radic Res

Apelin deficiency exacerbates cardiac injury following infarction by accelerating cardiomyocyte ferroptosis

Authors - Yuechu Zhao
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