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Validation Data Gallery

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  • Western Blot (WB) analysis of SKOV-3 cells using STK10 Polyclonal antibody (25471-1-AP)

    WB analysis of SKOV-3 using 25471-1-AP (same clone as 25471-1-PBS)

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 25471-1-AP (STK10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25471-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using STK10 Polyclonal antibody (25471-1-AP)

    WB analysis of A549 using 25471-1-AP (same clone as 25471-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 25471-1-AP (STK10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25471-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using STK10 Polyclonal antibody (25471-1-AP)

    WB analysis of A549 using 25471-1-AP (same clone as 25471-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 25471-1-AP (STK10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25471-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COLO 320 cells using STK10 Polyclonal antibody (25471-1-AP)

    WB analysis of COLO 320 using 25471-1-AP (same clone as 25471-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 25471-1-AP (STK10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25471-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using STK10 Polyclonal antibody (25471-1-AP)

    IHC staining of human lymphoma using 25471-1-AP (same clone as 25471-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 25471-1-AP (STK10 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25471-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using STK10 Polyclonal antibody (25471-1-AP)

    IHC staining of human lymphoma using 25471-1-AP (same clone as 25471-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 25471-1-AP (STK10 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25471-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

STK10 Polyclonal antibody, PBS Only

STK10 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 25471-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

EC:2.7.11.1, LOK, Lymphocyte oriented kinase, Lymphocyte-oriented kinase, PRO2729

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

25471-1-PBS targets STK10 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag22169

Product name: Recombinant human STK10 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 369-519 aa of BC070077

Sequence: SQSQDSVNEPCSQPSGDRSLQTTSPPVVAPGNENGLAVPVPLRKSRPVSMDARIQVAQEKQVAEQGGDLSPAANRSQKASQSRPNSSALETLGGEKLANGSLEPPAQAAPGPSKRDSDCSSLCTSESMDYGTNLSTDLSLNKEMGSLSIKD

相同性解析による交差性が予測される生物種
Full Name serine/threonine kinase 10
Calculated molecular weight 968 aa, 112 kDa
Observed molecular weight 130 kDa
GenBank accession numberBC070077
Gene Symbol STK10
Gene ID (NCBI) 6793
RRIDAB_2880096
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO94804
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Lymphocyte-oriented kinase deficiency encoded by the serine/threonine kinase 10 (STK10) gene correlates with the intracellular adhesion molecule 1 (ICAM-1)/lymphocyte function associated antigen 1 (LFA-1) complex in aspirin hypersensitivity(PMID:21905501). STK 10 is also named as LOK and expressed as a 130-kDa protein, which was detected predominantly in lymphoid organs such as spleen, thymus, and bone marrow, in contrast to other mammalian members of the STE20 family(PMID:9278426).

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