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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using STIL Monoclonal antibody (66876-1-Ig)

    WB analysis using 66876-1-Ig (same clone as 66876-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66876-1-Ig (STIL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66876-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using STIL Monoclonal antibody (66876-1-Ig)

    IHC staining of human colon cancer using 66876-1-Ig (same clone as 66876-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66876-1-Ig (STIL antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66876-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using STIL Monoclonal antibody (66876-1-Ig)

    IHC staining of human colon cancer using 66876-1-Ig (same clone as 66876-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66876-1-Ig (STIL antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66876-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using STIL Monoclonal antibody (66876-1-Ig)

    IHC staining of human lung cancer using 66876-1-Ig (same clone as 66876-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66876-1-Ig (STIL antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66876-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using STIL Monoclonal antibody (66876-1-Ig)

    IHC staining of human lung cancer using 66876-1-Ig (same clone as 66876-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66876-1-Ig (STIL antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66876-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using STIL Monoclonal antibody (66876-1-Ig)

    IF Staining of A431 using 66876-1-Ig (same clone as 66876-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using STIL antibody (66876-1-Ig, Clone: 1E12C1 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 66876-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using STIL Monoclonal antibody (66876-1-Ig)

    IF Staining of Jurkat using 66876-1-Ig (same clone as 66876-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using STIL antibody (66876-1-Ig, Clone: 1E12C1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 66876-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

STIL Monoclonal antibody, PBS Only

STIL Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66876-1-PBS
Clone No.1E12C1

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

1E12C1, SCL-interrupting locus protein, SIL, TAL-1-interrupting locus protein

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66876-1-PBS targets STIL in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag19124

Product name: Recombinant human STIL protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 928-1277 aa of BC126223

Sequence: LHQPSDNQKIYQDLLGQVNHLLNSSSKETEQPSTKAVIISHECTRTQNVYHTKKKTHHSRLVDKDCVLNATLKQLRSLGVKIDSPTKVKKNAHNVDHASVLACISPEAVISGLNCMSFANVGMSGLSPNGVDLSMEANAIALKYLNENQLSQLSVTRSNQNNCDPFSLLHINTDRSTVGLSLISPNNMSFATKKYMKRYGLLQSSDNSEDEEEPPDNADSKSEYLLNQNLRSIPEQLGGQKEPSKNDHEIINCSNCESVGTNADTPVLRNITNEVLQTKAKQQLTEKPAFLVKNLKPSPAVNLRTGKAEFTQHPEKENEGDITIFPESLQPSETLKQMNSMNSVGTFLDV

相同性解析による交差性が予測される生物種
Full Name SCL/TAL1 interrupting locus
Calculated molecular weight 1287 aa, 143 kDa
Observed molecular weight 150-155 kDa
GenBank accession numberBC126223
Gene Symbol STIL
Gene ID (NCBI) 6491
RRIDAB_2882210
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ15468
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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