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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using STEAP2 Polyclonal antibody (20201-1-AP)

    WB analysis of HEK-293 using 20201-1-AP (same clone as 20201-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 20201-1-AP (STEAP2-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20201-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using STEAP2 Polyclonal antibody (20201-1-AP)

    WB analysis of HeLa using 20201-1-AP (same clone as 20201-1-PBS)

    WB result of STEAP2-Specific antibody (20201-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-STEAP2-Specific transfected HeLa cells. This data was developed using the same antibody clone with 20201-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of DU 145 cells using STEAP2 Polyclonal antibody (20201-1-AP)

    WB analysis of DU 145 using 20201-1-AP (same clone as 20201-1-PBS)

    DU 145 cells were subjected to SDS PAGE followed by western blot with 20201-1-AP (STEAP2-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20201-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of fetal human brain tissue using STEAP2 Polyclonal antibody (20201-1-AP)

    WB analysis of fetal human brain using 20201-1-AP (same clone as 20201-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 20201-1-AP (STEAP2-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20201-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using STEAP2 Polyclonal antibody (20201-1-AP)

    WB analysis of human brain using 20201-1-AP (same clone as 20201-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 20201-1-AP (STEAP2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20201-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse kidney tissue using STEAP2 Polyclonal antibody (20201-1-AP)

    WB analysis of mouse kidney using 20201-1-AP (same clone as 20201-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20201-1-AP (STEAP2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20201-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

STEAP2 Polyclonal antibody, PBS Only

STEAP2 Polyclonal Antibody for WB, Indirect ELISA
Cat No. 20201-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Synonyms

STEAP2-Specific, EC:1.16.1.-, Metalloreductase STEAP2, PCANAP1, Prostate cancer-associated protein 1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20201-1-PBS targets STEAP2 in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name six transmembrane epithelial antigen of the prostate 2
Calculated molecular weight 56 kDa
Observed molecular weight 48-52 kDa
GenBank accession numberNM_152999
Gene Symbol STEAP2
Gene ID (NCBI) 261729
RRIDAB_10665556
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NFT2
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

STEAP2, also named as PCANAP1 and STAMP1, belongs to the STEAP family. It is a metalloreductase that has the ability to reduce both Fe3+ to Fe2+ and Cu2+ to Cu1+. STEAP2 uses NAD+ as acceptor. The antibody is specific to STEAP2.

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