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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

STEAP1 Polyclonal antibody

STEAP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20199-1-AP

Synonyms

Metalloreductase STEAP1, PRSS24, STEAP, STEAP1

Validation Data Gallery

Applications:
  • WB analysis of A431 using 20199-1-AP

    WB analysis of A431 using 20199-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 20199-1-AP (STEAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of A431 using 20199-1-AP

    WB analysis of A431 using 20199-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 20199-1-AP (STEAP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of DU 145 using 20199-1-AP

    WB analysis of DU 145 using 20199-1-AP

    DU 145 cells were subjected to SDS PAGE followed by western blot with 20199-1-AP (STEAP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 20199-1-AP

    IHC staining of human prostate cancer using 20199-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 20199-1-AP (STEAP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human prostate cancer using 20199-1-AP

    IHC staining of human prostate cancer using 20199-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 20199-1-AP (STEAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inA431 cells, DU 145 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

20199-1-AP targets STEAP1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen STEAP1 fusion protein Ag14134 相同性解析による交差性が予測される生物種
Full Name six transmembrane epithelial antigen of the prostate 1
Calculated molecular weight 339 aa, 40 kDa
Observed molecular weight 38-40 kDa
GenBank accession numberBC011802
Gene symbol STEAP1
Gene ID (NCBI) 26872
RRIDAB_11182488
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for STEAP1 antibody 20199-1-APDownload protocol
IHC protocol for STEAP1 antibody 20199-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Cell Death Dis

METTL3-mediated m6A modification of STEAP2 mRNA inhibits papillary thyroid cancer progress by blocking the Hedgehog signaling pathway and epithelial-to-mesenchymal transition.

Authors - Yue Zhu
View Article
humanWB

Cancer Biol Ther

STEAP2 promotes osteosarcoma progression by inducing epithelial–mesenchymal transition via the PI3K/AKT/mTOR signaling pathway and is regulated by EFEMP2

Authors - Dong Zhang
View Article