Validation Data Gallery
Tested Applications
| Positive IHC detected in | human renal cell carcinoma tissue, human lung cancer tissue, human colon cancer tissue, human kidney tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 10 publications below |
| IHC | See 10 publications below |
| IF | See 2 publications below |
Product Information
10314-1-AP targets Stanniocalcin 2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0359 Product name: Recombinant human STC2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 11-291 aa of BC000658 Sequence: TLALVLATFDPARGTDATNPPEGPQDRSSQQKGRLSLQNTAEIQHCLVNAGDVGCGVFECFENNSCEIRGLHGICMTFLHNAGKFDAQGKSFIKDALKCKAHALRHRFGCISRKCPAIREMVSQLQRECYLKHDLCAAAQENTRVIVEMIHFKDLLLHEPYVDLVNLLLTCGEEVKEAITHSVQVQCEQNWGSLCSILSFCTSAIQKPPTAPPERQPQVDRTKLSRAHHGEAGHHLPEPSSRETGRGAKGERGSKSHPNAHARGRVGGLGAQGPSGSSEWE 相同性解析による交差性が予測される生物種 |
| Full Name | stanniocalcin 2 |
| Calculated molecular weight | 33 kDa |
| GenBank accession number | BC000658 |
| Gene Symbol | Stanniocalcin 2 |
| Gene ID (NCBI) | 8614 |
| RRID | AB_2197546 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O76061 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Stanniocalcin (STC) is a family of secreted glycoprotein hormones that originally discovered in the corpuscles of Stannius, an endocrine gland of fish. STC1 and STC2, two homologues of STC family, are reported to involve in calcium and phosphate homeostasis. It is expressed in a wide variety of tissues such as kidney, spleen, heart, and pancreas. The protein may play a role in the regulation of renal and intestinal calcium and phosphate transport, cell metabolism, or cellular calcium/phosphate homeostasis. STC2 overexpression could promote tumor cell proliferation, invasion and metastasis in prostate cancer, ovarian cancer or neuroblastoma. STC2 is also vital for cytoprotective properties when exposed to ER stress and hypoxia.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Stanniocalcin 2 antibody 10314-1-AP | Download protocol |
| IHC protocol for Stanniocalcin 2 antibody 10314-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Transl Med Construction of an ER stress-related prognostic signature for predicting prognosis and screening the effective anti-tumor drug in osteosarcoma
| ||
Proc Natl Acad Sci U S A Addiction to Golgi-resident PI4P synthesis in chromosome 1q21.3-amplified lung adenocarcinoma cells. | ||
Genomics Proteomics Bioinformatics miRNA-mRNA Regulatory Network and Factors Associated with Prediction of Prognosis in Hepatocellular Carcinoma. | ||
Front Immunol An immune-related signature based on molecular subtypes for predicting the prognosis and immunotherapy efficacy of hepatocellular carcinoma |