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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using STBD1 Recombinant monoclonal antibody (87526-1-RR)

    WB analysis using 87526-1-RR (same clone as 87526-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87526-1-RR (STBD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87526-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using STBD1 Recombinant monoclonal antibody (87526-1-RR)

    IF Staining of HeLa using 87526-1-RR (same clone as 87526-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using STBD1 antibody (87526-1-RR, Clone: 252915D10 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 87526-1-PBS in a different storage buffer formulation.

  • Affinity of 87526-1-RR

    Affinity of 87526-1-RR

    Biolayer interferometry (BLl) kinetic assays of 87526-1-RR against Human STBD1 were performed. The affinity constant is 0.666 nM.

STBD1 Recombinant monoclonal antibody, PBS Only

STBD1 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 87526-1-PBS
Clone No.252915D10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Genethonin-1, GENX-3414, Glycophagy cargo receptor STBD1, starch binding domain 1, Starch-binding domain-containing protein 1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

87526-1-PBS targets STBD1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag2481

Product name: Recombinant human STBD1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 26-358 aa of BC022301

Sequence: GPGDTGKDGDAEQEKDAPLGGAAIPGGHQSGSSGLSPGPSGQELVTKPEHLQESNGHLISKTKDLGKLQAASWRLQNPSREVCDNSREHVPSGQFPDTEAPATSETSNSRSYSEVSRNESLESPMGEWGFQKGQEISAKAATCFAEKLPSSNLLKNRAKEEMSLSDLNSQDRVDHEEWEMVPRHSSWGDVGVGGSLKAPVLNLNQGMDNGRSTLVEARGQQVHGKMERVAVMPAGSQQVSVRFQVHYVTSTDVQFIAVTGDHECLGRWNTYIPLHYNKDGFWSHSIFLPADTVVEWKFVLVENGGVTRWEECSNRFLETGHEDKVVHAWWGIH

相同性解析による交差性が予測される生物種
Full Name starch binding domain 1
Calculated molecular weight 41 kDa
Observed molecular weight 35-43 kDa
GenBank accession numberBC022301
Gene Symbol STBD1
Gene ID (NCBI) 8987
RRIDAB_3745598
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDO95210
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

STBD1 may have the capability to bind to carbohydrates. It functions as a glycogen receptor that tethers glycogen to autophagic membranes for delivery and breakdown in lysosomes. STBD1 appears molecular mass of 35-38 kDa band in mouse/rat tissues and 38-43 kDa in human tissue.

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