Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse skeletal muscle tissue, HeLa cells, A549 cells |
| Positive IP detected in | A549 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 11 publications below |
| IHC | See 1 publications below |
| IF | See 7 publications below |
| IP | See 2 publications below |
Product Information
11842-1-AP targets STBD1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2481 Product name: Recombinant human STBD1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 26-358 aa of BC022301 Sequence: GPGDTGKDGDAEQEKDAPLGGAAIPGGHQSGSSGLSPGPSGQELVTKPEHLQESNGHLISKTKDLGKLQAASWRLQNPSREVCDNSREHVPSGQFPDTEAPATSETSNSRSYSEVSRNESLESPMGEWGFQKGQEISAKAATCFAEKLPSSNLLKNRAKEEMSLSDLNSQDRVDHEEWEMVPRHSSWGDVGVGGSLKAPVLNLNQGMDNGRSTLVEARGQQVHGKMERVAVMPAGSQQVSVRFQVHYVTSTDVQFIAVTGDHECLGRWNTYIPLHYNKDGFWSHSIFLPADTVVEWKFVLVENGGVTRWEECSNRFLETGHEDKVVHAWWGIH 相同性解析による交差性が予測される生物種 |
| Full Name | starch binding domain 1 |
| Calculated molecular weight | 41 kDa |
| Observed molecular weight | 35-43 kDa |
| GenBank accession number | BC022301 |
| Gene Symbol | STBD1 |
| Gene ID (NCBI) | 8987 |
| RRID | AB_2197523 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95210 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
STBD1 may have the capability to bind to carbohydrates. It functions as a glycogen receptor that tethers glycogen to autophagic membranes for delivery and breakdown in lysosomes. STBD1 appears molecular mass of 35-38 kDa band in mouse/rat tissues and 38-43 kDa in human tissue.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for STBD1 antibody 11842-1-AP | Download protocol |
| IP protocol for STBD1 antibody 11842-1-AP | Download protocol |
| WB protocol for STBD1 antibody 11842-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Redox Biol Dysfunction of astrocytic glycophagy exacerbates reperfusion injury in ischemic stroke | ||
Acta Pharmacol Sin Asiatic acid alleviates ischemic myocardial injury in mice by modulating mitophagy- and glycophagy-based energy metabolism. | ||
J Cell Sci Stbd1 promotes glycogen clustering during endoplasmic reticulum stress and supports survival of mouse myoblasts
| ||
Mol Endocrinol Transgenic muscle specific Nor-1 expression regulates multiple pathways that effect adiposity, metabolism and endurance. | ||
J Cell Sci Mouse Stbd1 is N-myristoylated and affects ER-mitochondria association and mitochondrial morphology. |
