Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells, HepG2 cells, K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84822-4-RR targets STAU1 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag5464 Product name: Recombinant human STAU1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 227-577 aa of BC050432 Sequence: GEGEGKSKKISKKNAAIAVLEELKKLPPLPAVERVKPRIKKKTKPIVKPQTSPEYGQGINPISRLAQIQQAKKEKEPEYTLLTERGLPRRREFVMQVKVGNHTAEGTGTNKKVAKRNAAENMLEILGFKVPQAQPTKPALKSEEKTPIKKPGDGRKVTFFEPGSGDENGTSNKEDEFRMPYLSHQQLPAGILPMVPEVAQAVGVSQGHHTKDFTRAAPNPAKATVTAMIARELLYGGTSPTAETILKNNISSGHVPHGPLTRPSEQLDYLSRVQGFQVEYKDFPKNNKNEFVSLINCSSQPPLISHGIGKDVESCHDMAALNILKLLSELDQQSTEMPRTGNGPMSVCGRC 相同性解析による交差性が予測される生物種 |
| Full Name | staufen, RNA binding protein, homolog 1 (Drosophila) |
| Calculated molecular weight | 63 kDa |
| Observed molecular weight | 55-65 kDa |
| GenBank accession number | BC050432 |
| Gene Symbol | STAU1 |
| Gene ID (NCBI) | 6780 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | O95793 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Staufen1 (STAU1)-mediated mRNA decay (SMD) degrades translationally active mRNAs that bind the double-stranded (ds)RNA binding protein STAU1 within their 3'-untranslated regions (3'UTRs) [PMID:21307942]. STAU1 can binds to stem structures and protect Dvl2 mRNA from degradation by associating with the secondary structure in the U1 region which is located at 5′ position of second U stretch in the U2 region [PMID:17510634]. It has a role in specific positioning of mRNAs at given sites in the cell by cross-linking cytoskeletal and RNA components, and in stimulating their translation at the site.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for STAU1 antibody 84822-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |