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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using STAT6 Recombinant antibody (82630-1-RR)

    WB analysis using 82630-1-RR (same clone as 82630-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82630-1-RR (STAT6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82630-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using STAT6 Recombinant antibody (82630-1-RR)

    WB analysis of HeLa using 82630-1-RR (same clone as 82630-1-PBS)

    WB result of STAT6 antibody (82630-1-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STAT6 transfected HeLa cells. This data was developed using the same antibody clone with 82630-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using STAT6 Recombinant antibody (82630-1-RR)

    IHC staining of human colon cancer using 82630-1-RR (same clone as 82630-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82630-1-RR (STAT6 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82630-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using STAT6 Recombinant antibody (82630-1-RR)

    IHC staining of human stomach using 82630-1-RR (same clone as 82630-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 82630-1-RR (STAT6 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82630-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using STAT6 Recombinant antibody (82630-1-RR)

    IHC staining of mouse spleen using 82630-1-RR (same clone as 82630-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 82630-1-RR (STAT6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82630-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using STAT6 Recombinant antibody (82630-1-RR)

    IHC staining of mouse spleen using 82630-1-RR (same clone as 82630-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 82630-1-RR (STAT6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82630-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using STAT6 Recombinant antibody (82630-1-RR)

    IF Staining of HeLa using 82630-1-RR (same clone as 82630-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using STAT6 antibody (82630-1-RR, Clone: 4F19 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82630-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

STAT6 Recombinant monoclonal antibody, PBS Only

STAT6 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 82630-1-PBS
Clone No.4F19

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

4F19, IL-4 Stat, Signal transducer and activator of transcription 6, STAT 6, STAT6B

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82630-1-PBS targets STAT6 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name signal transducer and activator of transcription 6, interleukin-4 induced
Calculated molecular weight 94 kDa
Observed molecular weight105-110 kDa
GenBank accession numberBC075852
Gene Symbol STAT6
Gene ID (NCBI) 6778
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP42226
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

STAT6, also named as Signal transducer and activator of transcription 6, is a 847 amino acid protein, which belongs to the transcription factor STAT family. STAT6 forms a homodimer or a heterodimer with a related family member. STAT6 translocates into the nucleus in response to phosphorylation. STAT6 is Involved in IL4/interleukin-4- and IL3/interleukin-3-mediated signaling.

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