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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using STAT6 Monoclonal antibody (66717-1-Ig)

    WB analysis using 66717-1-Ig (same clone as 66717-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66717-1-Ig (STAT6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66717-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using STAT6 Monoclonal antibody (66717-1-Ig)

    WB analysis of HeLa using 66717-1-Ig (same clone as 66717-1-PBS)

    WB result of STAT6 antibody (66717-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STAT6 transfected HeLa cells. This data was developed using the same antibody clone with 66717-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using STAT6 Monoclonal antibody (66717-1-Ig)

    WB analysis using 66717-1-Ig (same clone as 66717-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66717-1-Ig (STAT6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66717-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using STAT6 Monoclonal antibody (66717-1-Ig)

    IHC staining of human lymphoma using 66717-1-Ig (same clone as 66717-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66717-1-Ig (STAT6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66717-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using STAT6 Monoclonal antibody (66717-1-Ig)

    IHC staining of human appendicitis using 66717-1-Ig (same clone as 66717-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66717-1-Ig (STAT6 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66717-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using STAT6 Monoclonal antibody (66717-1-Ig)

    IHC staining of human lymphoma using 66717-1-Ig (same clone as 66717-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66717-1-Ig (STAT6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66717-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using STAT6 Monoclonal antibody (66717-1-Ig)

    IHC staining of human appendicitis using 66717-1-Ig (same clone as 66717-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66717-1-Ig (STAT6 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66717-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

STAT6 Monoclonal antibody, PBS Only

STAT6 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66717-1-PBS
Clone No.2E5B8

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

2E5B8, IL-4 Stat, Signal transducer and activator of transcription 6, STAT 6, STAT6B

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66717-1-PBS targets STAT6 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag0410

Product name: Recombinant human STAT6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 368-567 aa of BC005823

Sequence: IKRCERKGTESVTEEKCAVLFSASFTLGPGKLPIQLQALSLPLVVIVHGNQDNNAKATILWYNAFSEMDRVPFVVAERVPWEKMCETLNLKFMAEVGTNRGLLPEHFLFLAQKIFNDNSLSMEAFQHRSVSWSQFNKEILLGRGFTFWQWFDGVLDLTKRCLRSYWSDRLIIGFISKQYVTSLLLNEPDGTFLLRFSDSE

相同性解析による交差性が予測される生物種
Full Name signal transducer and activator of transcription 6, interleukin-4 induced
Calculated molecular weight 100 kDa
Observed molecular weight 95 kDa
GenBank accession numberBC005823
Gene Symbol STAT6
Gene ID (NCBI) 6778
RRIDAB_2882068
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP42226
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

STAT6, also named as Signal transducer and activator of transcription 6, is a 847 amino acid protein, which belongs to the transcription factor STAT family. STAT6 forms a homodimer or a heterodimer with a related family member. STAT6 translocates into the nucleus in response to phosphorylation. STAT6 is Involved in IL4/interleukin-4- and IL3/interleukin-3-mediated signaling.

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