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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using STAT4 Monoclonal antibody (67568-2-Ig)

    WB analysis using 67568-2-Ig (same clone as 67568-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67568-2-Ig (STAT4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67568-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of 4T1 cells using STAT4 Monoclonal antibody (67568-2-Ig)

    WB analysis of 4T1 using 67568-2-Ig (same clone as 67568-2-PBS)

    4T1 cells were subjected to SDS PAGE followed by western blot with 67568-2-Ig (STAT4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67568-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using STAT4 Monoclonal antibody (67568-2-Ig)

    WB analysis using 67568-2-Ig (same clone as 67568-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67568-2-Ig (STAT4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67568-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using STAT4 Monoclonal antibody (67568-2-Ig)

    IHC staining of human breast cancer using 67568-2-Ig (same clone as 67568-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67568-2-Ig (STAT4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67568-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using STAT4 Monoclonal antibody (67568-2-Ig)

    IHC staining of human breast cancer using 67568-2-Ig (same clone as 67568-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67568-2-Ig (STAT4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67568-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using STAT4 Monoclonal antibody (67568-2-Ig)

    IHC staining of human cervical cancer using 67568-2-Ig (same clone as 67568-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67568-2-Ig (STAT4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67568-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using STAT4 Monoclonal antibody (67568-2-Ig)

    IHC staining of human cervical cancer using 67568-2-Ig (same clone as 67568-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67568-2-Ig (STAT4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67568-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using STAT4 Monoclonal antibody (67568-2-Ig)

    IF Staining of HepG2 using 67568-2-Ig (same clone as 67568-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using STAT4 antibody (67568-2-Ig, Clone: 4A8C9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67568-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using STAT4 Monoclonal antibody (67568-2-Ig)

    FC experiment of HepG2 using 67568-2-Ig (same clone as 67568-2-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug STAT4 Monoclonal antibody (67568-2-Ig, Clone:4A8C9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67568-2-PBS in a different storage buffer formulation.

STAT4 Monoclonal antibody, PBS Only

STAT4 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 67568-2-PBS
Clone No.4A8C9

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

4A8C9, DPMC, SLEB11

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67568-2-PBS targets STAT4 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag19545

Product name: Recombinant human STAT4 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 399-748 aa of BC031212

Sequence: FRHLQPKEMKSSAGGKGNEGCHMVTEELHSITFETQICLYGLTIDLETSSLPVVMISNVSQLPNAWASIIWYNVSTNDSQNLVFFNNPPPATLSQLLEVMSWQFSSYVGRGLNSDQLHMLAEKLTVQSSYSDGHLTWAKFCKEHLPGKSFTFWTWLEAILDLIKKHILPLWIDGYVMGFVSKEKERLLLKDKMPGTFLLRFSESHLGGITFTWVDHSESGEVRFHSVEPYNKGRLSALPFADILRDYKVIMAENIPENPLKYLYPDIPKDKAFGKHYSSQPCEVSRPTERGDKGYVPSVFIPISTIRSDSTEPHSPSDLLPMSPSVYAVLRENLSPTTIETAMKSPYSAE

相同性解析による交差性が予測される生物種
Full Name signal transducer and activator of transcription 4
Calculated molecular weight 748 aa, 86 kDa
Observed molecular weight 86 kDa
GenBank accession numberBC031212
Gene Symbol STAT4
Gene ID (NCBI) 6775
RRIDAB_2882782
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ14765
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The JAK/STAT pathway is an extensive signaling pathway downstream of cytokine receptors. STATs are cytosolic proteins with a common structure consisting of an N-terminal oligomerization domain, which favors formation of STAT dimers, followed by a DNA-binding domain and a C-terminal SRC homology-2 (SH2) domain, which is involved in association between STATs and receptors[PMID:22383755]. Signal Transducer and Activator of Transcription 4 (STAT4) is a transcription factor that is activated by IL-12 signaling and promotes Th1-cell differentiation and IFN-γ production [PMID:21998209].

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