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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Phospho-STAT1 (Ser727) Recombinant antibody

Phospho-STAT1 (Ser727) Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240897D10

Cat no : 80683-7-RR

Synonyms

phospho-S727 STAT1, Phospho STAT1 (Ser727), Phospho STAT1, p Stat1 (S727), phospho-STAT1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-STAT1 (Ser727) Recombinant antibody (80683-7-RR)

    WB analysis using 80683-7-RR

    Non treated and IFN gamma treated HeLa cells were subjected to SDS PAGE followed by western blot with 80683-7-RR (Phospho-STAT1 (Ser727) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

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Tested Applications

Positive WB detected inIFN gamma treated HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

80683-7-RR targets Phospho-STAT1 (Ser727) in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name signal transducer and activator of transcription 1, 91kDa
Calculated molecular weight 83 kDa
Observed molecular weight84-91 kDa
GenBank accession numberBC002704
Gene symbol STAT1
Gene ID (NCBI) 6772
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

STAT1 (signal transducers and activators of transcription 1) is a member of the STAT protein family. STAT family members are phosphorylated by the receptor associated kinases, and then form homo-or heterodimers that translocate to the cell nucleus where they act as transcription activators. The phosphorylation of STAT1 at Tyr701 promotes the dimerization of STAT1 and its translocation into the nucleus where it binds the GAS promoter to allow the transcription of inflammatory response genes(PMID: 31408005). The transcript of STAT1 undergoes alternative splicing, resulting in two isoforms: STAT1α (91 kDa) and STAT1β (84 kDa)(PMID: 20159032).

Protocols

Product Specific Protocols
WB protocol for Phospho-STAT1 (Ser727) antibody 80683-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols