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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using STARD7 Recombinant antibody (85131-4-RR)

    WB analysis using 85131-4-RR (same clone as 85131-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85131-4-RR (STARD7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85131-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using STARD7 Recombinant antibody (85131-4-RR)

    IHC staining of human colon cancer using 85131-4-RR (same clone as 85131-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85131-4-RR (STARD7 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85131-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using STARD7 Recombinant antibody (85131-4-RR)

    IHC staining of human colon cancer using 85131-4-RR (same clone as 85131-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85131-4-RR (STARD7 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85131-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using STARD7 Recombinant antibody (85131-4-RR)

    IHC staining of human colon cancer using 85131-4-RR (same clone as 85131-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85131-4-RR (STARD7 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85131-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85131-4-RR

    Affinity and Kinetic Characterization of 85131-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85131-4-RR against Human STARD7 were performed. The affinity constant is below 1 pM.

STARD7 Recombinant monoclonal antibody, PBS Only

STARD7 Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 85131-4-PBS
Clone No.242760A3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

242760A3, GTT1, StAR-related lipid transfer protein 7, mitochondrial, START domain-containing protein 7

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85131-4-PBS targets STARD7 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag8224

Product name: Recombinant human STARD7 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-295 aa of BC008894

Sequence: MAALAGVFVWDEERIQEEELQRSINEMKRLEEMSNMFQSSGVQHHPPEPKAQTEGNEDSEGKEQRWEMVMDKKHFKLWRRPITGTHLYQYRVFGTYTDVTPRQFFNVQLDTEYRKKWDALVIKLEVIERDVVSGSEVLHWVTHFPYPMYSRDYVYVRRYSVDQENNMMVLVSRAVEHPSVPESPEFVRVRSYESQMVIRPHKSFDENGFDYLLTYSDNPQTVFPRYCVSWMVSSGMPDFLEKLHMATLKAKNMEIKVKDYISAKPLEMSSEAKATSQSSERKNEGSCGPARIEYA

相同性解析による交差性が予測される生物種
Full Name StAR-related lipid transfer (START) domain containing 7
Calculated molecular weight 295 aa, 35 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC008894
Gene Symbol STARD7
Gene ID (NCBI) 56910
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9NQZ5
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

STARD7, also known as gestational trophoblastic tumor gene-1 (GTT1), is a lipid transfer protein that specifically binds and transports phosphatidylcholine (PC) between cellular membranes. It is a member of the START (Steroidogenic Acute Regulatory protein-related lipid transfer) domain-containing protein family, which is involved in intracellular lipid transport and metabolism. (PMID: 23507753)

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