Validation Data Gallery
Tested Applications
| Positive WB detected in | SK-BR-3 cells, MCF-7 cells, T-47D cells, LNCaP cells, rat liver tissue, mouse liver tissue, pig liver tissue, rabbit liver tissue, rabbit testis tissue |
| Positive IP detected in | HepG2 cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68554-1-Ig targets STARD10 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
| Tested Reactivity | human, mouse, rat, pig, rabbit |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10932 Product name: Recombinant human STARD10 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-291 aa of BC007919 Sequence: MEKLAASTEPQGPRPVLGRESVQVPDDQDFRSFRSECEAEVGWNLTYSRAGVSVWVQAVEMDRTLHKIKCRMECCDVPAETLYDVLHDIEYRKKWDSNVIETFDIARLTVNADVGYYSWRCPKPLKNRDVITLRSWLPMGADYIIMNYSVKHPKYPPRKDLVRAVSIQTGYLIQSTGPKSCVITYLAQVDPKGSLPKWVVNKSSQFLAPKAMKKMYKACLKYPEWKQKHLPHFKPWLHPEQSPLPSLALSELSVQHADSLENIDESAVAESREERMGGAGGEGSDDDTSLT 相同性解析による交差性が予測される生物種 |
| Full Name | StAR-related lipid transfer (START) domain containing 10 |
| Calculated molecular weight | 291 aa, 33 kDa |
| Observed molecular weight | 35-40 kDa |
| GenBank accession number | BC007919 |
| Gene Symbol | STARD10 |
| Gene ID (NCBI) | 10809 |
| RRID | AB_3085257 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9Y365 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
STARD10 (START domain-containing protein 10, also known as PCTP-like protein) is highly expressed in liver and responsible to transfer phosphatidylcholine. STARD10 was identified as a protein overexpressed in breast cancer that cooperates with the ErbB family of receptor tyrosine kinases in cellular transformation (PMID: 15911624). This antibody detected duplicate bands between 35-40 kDa (PMID: 15150109).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for STARD10 antibody 68554-1-Ig | Download protocol |
| IF protocol for STARD10 antibody 68554-1-Ig | Download protocol |
| IP protocol for STARD10 antibody 68554-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |