Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse testis tissue, human adrenal gland tissue, K-562 cells, pig adrenal gland tissue, rat ovary tissue, rat testis tissue |
| Positive IHC detected in | mouse testis tissue, human liver cancer tissue, human ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse testis tissue |
| Positive IF-Fro detected in | mouse testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 70 publications below |
| IHC | See 13 publications below |
| IF | See 17 publications below |
Product Information
12225-1-AP targets STAR in WB, IHC, IF-P, IF-Fro, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse, rat, pig, monkey, chicken, goat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2862 Product name: Recombinant human STAR protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-285 aa of BC010550 Sequence: MLLATFKLCAGSSYRHMRNMKGLRQQAVMAISQELNRRALGGPTPSTWINQVRRRSSLLGSRLEETLYSDQELAYLQQGEEAMQKALGILSNQEGWKKESQQDNGDKVMSKVVPDVGKVFRLEVVVDQPMERLYEELVERMEAMGEWNPNVKEIKVLQKIGKDTFITHELAAEAAGNLVGPRDFVSVRCAKRRGSTCVLAGMATDFGNMPEQKGVIRAEHGPTCMVLHPLAGSPSKTKLTWLLSIDLKGWLPKSIINQVLSQTQVDFANHLRKRLESHPASEARC 相同性解析による交差性が予測される生物種 |
| Full Name | steroidogenic acute regulatory protein |
| Calculated molecular weight | 285 aa, 32 kDa |
| Observed molecular weight | 26-30 kDa |
| GenBank accession number | BC010550 |
| Gene Symbol | STAR |
| Gene ID (NCBI) | 6770 |
| RRID | AB_2115832 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P49675 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Steroidogenic acute regulatory protein (StAR) is a key player in acute regulation of hormone-induced steroidogenesis, by facilitating cholesterol transport from cellular stores to inner mitochondria membrane where the cholesterol was conversed to pregnenolone. StAR is prevalently expressed in mitochondria of steroid-producing adrenal and gonadal tissue.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for STAR antibody 12225-1-AP | Download protocol |
| IHC protocol for STAR antibody 12225-1-AP | Download protocol |
| WB protocol for STAR antibody 12225-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Transl Res Induction of autophagy by Beclin-1 in granulosa cells contributes to follicular progesterone elevation in ovarian endometriosis. | ||
Clin Transl Med Single-cell transcriptomes reveal heterogeneity of high-grade serous ovarian carcinoma. | ||
Int J Nanomedicine La2O3 Nanoparticles Induce Reproductive Toxicity Mediated by the Nrf-2/ARE Signaling Pathway in Kunming Mice. | ||
Ecotoxicol Environ Saf FDX1 regulates leydig cell ferroptosis mediates PM2.5-induced testicular dysfunction of mice | ||
Environ Pollut Prenatal exposure to propylparaben at human-relevant doses accelerates ovarian aging in adult mice. | ||
Biol Res Unraveling the impact of hyperleptinemia on female reproduction: insights from transgenic pig model |