×
Use Able AI chat for product recommendations

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using STAM Polyclonal antibody (12434-1-AP)

    WB analysis using 12434-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12434-1-AP (STAM antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Multi-cells using STAM Polyclonal antibody (12434-1-AP)

    WB analysis of Multi-cells using 12434-1-AP

    WB result of 12434-1-aP (STAM antibody).

  • Western Blot (WB) analysis of mouse brain tissue using STAM Polyclonal antibody (12434-1-AP)

    WB analysis of mouse brain using 12434-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 12434-1-AP (STAM antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Y79 cells using STAM Polyclonal antibody (12434-1-AP)

    WB analysis of Y79 using 12434-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 12434-1-AP (STAM antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of PC-3 cells using STAM Polyclonal antibody (12434-1-AP)

    IP experiment of PC-3 using 12434-1-AP

    IP result of anti-STAM (IP:12434-1-AP, 4ug; Detection:12434-1-AP 1:300) with PC-3 cells lysate 1440ug.

  • Immunohistochemistry (IHC) staining of human testis tissue using STAM Polyclonal antibody (12434-1-AP)

    IHC staining of human testis using 12434-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12434-1-AP (STAM antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using STAM Polyclonal antibody (12434-1-AP)

    IHC staining of human testis using 12434-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12434-1-AP (STAM antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using STAM Polyclonal antibody (12434-1-AP)

    IHC staining of mouse testis using 12434-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12434-1-AP (STAM antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using STAM Polyclonal antibody (12434-1-AP)

    IHC staining of mouse testis using 12434-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12434-1-AP (STAM antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using STAM Polyclonal antibody (12434-1-AP)

    IHC staining of mouse kidney using 12434-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12434-1-AP (STAM antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using STAM Polyclonal antibody (12434-1-AP)

    IHC staining of human appendicitis using 12434-1-AP

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 12434-1-AP (STAM antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human rectal cancer tissue using STAM Polyclonal antibody (12434-1-AP)

    IHC staining of human rectal cancer using 12434-1-AP

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 12434-1-AP (STAM antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using STAM Polyclonal antibody (12434-1-AP)

    IHC staining of mouse cerebellum using 12434-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 12434-1-AP (STAM antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using STAM Polyclonal antibody (12434-1-AP)

    IHC staining of mouse small intestine using 12434-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 12434-1-AP (STAM antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat kidney tissue using STAM Polyclonal antibody (12434-1-AP)

    IHC staining of rat kidney using 12434-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 12434-1-AP (STAM antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat small intestine tissue using STAM Polyclonal antibody (12434-1-AP)

    IHC staining of rat small intestine using 12434-1-AP

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 12434-1-AP (STAM antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human rectal cancer tissue using STAM Polyclonal antibody (12434-1-AP)

    IHC staining of human rectal cancer using 12434-1-AP

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 12434-1-AP (STAM antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using STAM Polyclonal antibody (12434-1-AP)

    IF Staining of HepG2 using 12434-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using STAM antibody (12434-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using STAM Polyclonal antibody (12434-1-AP)

    IF Staining of HepG2 using 12434-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using STAM antibody (12434-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of HeLa cells using STAM Polyclonal antibody (12434-1-AP)

    FC experiment of HeLa using 12434-1-AP

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug STAM Polyclonal antibody (12434-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

  • Featured Product
  • KD/KO Validated

STAM Polyclonal antibody

STAM Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 12434-1-AP
Publications(22)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Synonyms

STAM 1, STAM1, STAM-1

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293T cells, Multi-cells, mouse brain tissue, Y79 cells, Jurkat cells, K-562 cells, MCF-7 cells, PC-3 cells, Raji cells, SKOV-3 cells, C2C12 cells
Positive IP detected inPC-3 cells
Positive IHC detected inhuman testis tissue, human appendicitis tissue, rat small intestine tissue, mouse small intestine tissue, mouse testis tissue, mouse cerebellum tissue, rat kidney tissue, human rectal cancer tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12434-1-AP targets STAM in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3112

Product name: Recombinant human STAM protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 54-403 aa of BC030586

Sequence: VNHKDPHVAMQALTLLGACVSNCGKIFHLEVCSRDFASEVSNVLNKGHPKVCEKLKALMVEWTDEFKNDPQLSLISAMIKNLKEQGVTFPAIGSQAAEQAKASPALVAKDPGTVANKKEEEDLAKAIELSLKEQRQQSTTLSTLYPSTSSLLTNHQHEGRKVRAIYDFEAAEDNELTFKAGEIITVLDDSDPNWWKGETHQGIGLFPSNFVTAYLTAEPEMIKTEKKTVQFSDDVQVETIEPEPEPAFIDEDKMDQLLQMLQSTDPSDDQPDLPELLHLEAMCHQMGPLIDEKLEDIDRKHSELSELNVKVMEALSLYTKLMNEDPMYSMYAKLQNQPGSGPTIRKPSPS

相同性解析による交差性が予測される生物種
Full Name signal transducing adaptor molecule (SH3 domain and ITAM motif) 1
Calculated molecular weight 59 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC030586
Gene Symbol STAM
Gene ID (NCBI) 8027
RRIDAB_2199965
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92783
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

STAM, also named as STAM1, belongs to the STAM family. It is involved in intracellular signal transduction mediated by cytokines and growth factors. Upon IL-2 and GM-CSL stimulation, it plays a role in signaling leading to DNA synthesis and MYC induction. STAM may also play a role in T-cell development. It is involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs). Together with HRS, STAM forms ESCRT-0. STAM was originally identified as an adaptor protein involved in cytokine signaling.(PMID:20505072)

Protocols

Product Specific Protocols
FC protocol for STAM antibody 12434-1-APDownload protocol
IF protocol for STAM antibody 12434-1-APDownload protocol
IHC protocol for STAM antibody 12434-1-APDownload protocol
IP protocol for STAM antibody 12434-1-APDownload protocol
WB protocol for STAM antibody 12434-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

A phosphoinositide signalling pathway mediates rapid lysosomal repair

Authors - Jay Xiaojun Tan
View Article
humanIF

Nat Cell Biol

Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation.

Authors - Vincent Mercier
View Article
WB,IF

Nat Commun

Mapping of endosomal proximity proteomes reveals Retromer as a hub for RAB GTPase regulation

Authors - Carlos Antón-Plágaro
View Article
humanWB

Mol Cell

Systematically defining selective autophagy receptor-specific cargo using autophagosome content profiling.

Authors - Susanne Zellner
View Article
humanWB

Sci Adv

A ubiquitin-specific, proximity-based labeling approach for the identification of ubiquitin ligase substrates

Authors - Urbi Mukhopadhyay
View Article
humanWB,IF

Cell Rep

TIM-1 Ubiquitination Mediates Dengue Virus Entry.

Authors - Ophélie Dejarnac
  • KD Validated
View Article
||
New chat

Able™

正在加载，请稍候...