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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using STAG2 Polyclonal antibody (19837-1-AP)

    WB analysis of Jurkat using 19837-1-AP (same clone as 19837-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 19837-1-AP (STAG2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19837-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using STAG2 Polyclonal antibody (19837-1-AP)

    WB analysis of MCF-7 using 19837-1-AP (same clone as 19837-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 19837-1-AP (STAG2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19837-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using STAG2 Polyclonal antibody (19837-1-AP)

    WB analysis of NIH/3T3 using 19837-1-AP (same clone as 19837-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 19837-1-AP (STAG2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19837-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using STAG2 Polyclonal antibody (19837-1-AP)

    WB analysis of HeLa using 19837-1-AP (same clone as 19837-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 19837-1-AP (STAG2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19837-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using STAG2 Polyclonal antibody (19837-1-AP)

    WB analysis of K-562 using 19837-1-AP (same clone as 19837-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 19837-1-AP (STAG2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19837-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using STAG2 Polyclonal antibody (19837-1-AP)

    IP experiment of MCF-7 using 19837-1-AP (same clone as 19837-1-PBS)

    IP result of anti-STAG2 (IP:19837-1-AP, 4ug; Detection:19837-1-AP 1:3000) with MCF-7 cells lysate 2120 ug. This data was developed using the same antibody clone with 19837-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of U-251 cells using STAG2 Polyclonal antibody (19837-1-AP)

    IP experiment of U-251 using 19837-1-AP (same clone as 19837-1-PBS)

    IP result of anti-STAG2 (IP:19837-1-AP, 4ug; Detection:19837-1-AP 1:3000) with U-251 cells lysate 3320 ug. This data was developed using the same antibody clone with 19837-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using STAG2 Polyclonal antibody (19837-1-AP)

    IHC staining of human cervical cancer using 19837-1-AP (same clone as 19837-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 19837-1-AP (STAG2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 19837-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using STAG2 Polyclonal antibody (19837-1-AP)

    IHC staining of human cervical cancer using 19837-1-AP (same clone as 19837-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 19837-1-AP (STAG2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 19837-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using STAG2 Polyclonal antibody (19837-1-AP)

    IF Staining of HEK-293 using 19837-1-AP (same clone as 19837-1-PBS)

    Immunofluorescent analysis of 293 cells, using STAG2 antibody 19837-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 19837-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using STAG2 Polyclonal antibody (19837-1-AP)

    IF Staining of HEK-293 using 19837-1-AP (same clone as 19837-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using STAG2 antibody (19837-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 19837-1-PBS in a different storage buffer formulation.

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STAG2 Polyclonal antibody, PBS Only

STAG2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 19837-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Stromal antigen 2, SA2, SA 2, Cohesin subunit SA-2, Cohesin subunit SA 2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

19837-1-PBS targets STAG2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen STAG2 fusion protein Ag13825 相同性解析による交差性が予測される生物種
Full Name stromal antigen 2
Calculated molecular weight 1231 aa, 141 kDa
Observed molecular weight 130-141 kDa
GenBank accession numberBC001765
Gene Symbol STAG2
Gene ID (NCBI) 10735
RRIDAB_10666847
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N3U4
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

STAG2, also named as SA2, is a component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex may play a role in spindle pole assembly during mitosis. Mutations in STAG2 appear to be a first step in the transformation of a normal cell into a cancer cell.