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Validation Data Gallery

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  • Western Blot (WB) analysis of human heart tissue using STAG1 Polyclonal antibody (14015-1-AP)

    WB analysis of human heart using 14015-1-AP (same clone as 14015-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 14015-1-AP (STAG1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14015-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Raji cells using STAG1 Polyclonal antibody (14015-1-AP)

    WB analysis of Raji using 14015-1-AP (same clone as 14015-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 14015-1-AP (STAG1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14015-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using STAG1 Polyclonal antibody (14015-1-AP)

    WB analysis using 14015-1-AP (same clone as 14015-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14015-1-AP (STAG1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14015-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using STAG1 Polyclonal antibody (14015-1-AP)

    WB analysis of HeLa using 14015-1-AP (same clone as 14015-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 14015-1-AP (STAG1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14015-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using STAG1 Polyclonal antibody (14015-1-AP)

    IHC staining of human stomach using 14015-1-AP (same clone as 14015-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 14015-1-AP (STAG1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14015-1-PBS in a different storage buffer formulation.

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STAG1 Polyclonal antibody, PBS Only

STAG1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 14015-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

His SA, Stromal antigen 1, SCC3, SA1, Cohesin subunit SA-1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14015-1-PBS targets STAG1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen STAG1 fusion protein Ag5123 相同性解析による交差性が予測される生物種
Full Name stromal antigen 1
Calculated molecular weight 144 kDa
Observed molecular weight 155 kDa
GenBank accession numberBC064699
Gene Symbol STAG1
Gene ID (NCBI) 10274
RRIDAB_2194759
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WVM7
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

STAG1, also named as SCC3 homolog 1, is a 1258 amino acid protein, which belongs to the SCC3 family. STAG1 interacts directly with RAD21 in cohesin complex and localizes in nucleus. STAG1 is a Component of cohesin complex, which is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. The calculate molecular weight of STAG1 is 144 kDa, but modified STAG1 is about 145-150 kDa.