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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ST8SIA3 Polyclonal antibody (18004-1-AP)

    WB analysis using 18004-1-AP (same clone as 18004-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18004-1-AP (ST8SIA3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18004-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using ST8SIA3 Polyclonal antibody (18004-1-AP)

    WB analysis of mouse liver using 18004-1-AP (same clone as 18004-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 18004-1-AP (ST8SIA3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18004-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using ST8SIA3 Polyclonal antibody (18004-1-AP)

    WB analysis of mouse liver using 18004-1-AP (same clone as 18004-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 18004-1-AP (ST8SIA3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18004-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using ST8SIA3 Polyclonal antibody (18004-1-AP)

    IHC staining of human liver using 18004-1-AP (same clone as 18004-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 18004-1-AP (ST8SIA3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 18004-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using ST8SIA3 Polyclonal antibody (18004-1-AP)

    IHC staining of human liver using 18004-1-AP (same clone as 18004-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 18004-1-AP (ST8SIA3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 18004-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin tissue using ST8SIA3 Polyclonal antibody (18004-1-AP)

    IHC staining of human skin using 18004-1-AP (same clone as 18004-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 18004-1-AP (ST8SIA3 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 18004-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin tissue using ST8SIA3 Polyclonal antibody (18004-1-AP)

    IHC staining of human skin using 18004-1-AP (same clone as 18004-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 18004-1-AP (ST8SIA3 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 18004-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18004-1-PBS targets ST8SIA3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ST8SIA3 fusion protein Ag12497 相同性解析による交差性が予測される生物種
Full Name ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 3
Calculated molecular weight 380 aa, 44 kDa
Observed molecular weight 44 kDa
GenBank accession numberBC074909
Gene Symbol ST8SIA3
Gene ID (NCBI) 51046
RRIDAB_2239838
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43173
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The ST8 α-N-acetyl-neuraminide α-2,8-sialyltransferase (ST8Sia) family catalyzes the synthesis of one or multiple α2,8-linked sialic acid chains according to their acceptor specifificity (PMID:19888308). At least fifive ST8Sia enzymes (ST8SIA1, 2, 3, 4, and 6) have previously been identifified in adult brains (PMID:11530204). The ST8SIA3 is enriched in the striatum. The mouse St8sia3 gene shares 96% amino acid identity with human ST8SIA3, and is mainly expressed in the brain and testis (PMID:31455764).