Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse heart tissue |
| Positive IHC detected in | human heart tissue, human skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IHC | See 2 publications below |
| IF | See 2 publications below |
Product Information
19736-1-AP targets ST8SIA2-Specific in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 2 |
| Calculated molecular weight | 42 kDa |
| Observed molecular weight | 42 kDa |
| GenBank accession number | NM_006011 |
| Gene Symbol | ST8SIA2 |
| Gene ID (NCBI) | 8128 |
| RRID | AB_10638471 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q92186 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ST8SIA2, also named as SIAT8B and STX, belongs to the glycosyltransferase 29 family. ST8SIA2 may transfer sialic acid through alpha-2,8-linkages to the alpha-2,3-linked and alpha-2,6-linked sialic acid of N-linked oligosaccharides of glycoproteins and may be involved in PSA (polysialic acid) expression. The antibody is specific to ST8SIA2.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ST8SIA2-Specific antibody 19736-1-AP | Download protocol |
| IHC protocol for ST8SIA2-Specific antibody 19736-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Neurosci Single-cell genomics of the mouse olfactory cortex reveals contrasts with neocortex and ancestral signatures of cell type evolution | ||
Oxid Med Cell Longev Long Noncoding RNA TUG1 Aggravates Cerebral Ischemia/Reperfusion Injury by Acting as a ceRNA for miR-3072-3p to Target St8sia2. | ||
J Cell Sci Prolyl endopeptidase is involved in the degradation of neural cell adhesion molecules in vitro. | ||
Brain Res PSA modification of NCAM supports the survival of injured retinal ganglion cells in adulthood. | ||
Front Immunol A novel ganglioside-related risk signature can reveal the distinct immune landscape of neuroblastoma and predict the immunotherapeutic response |