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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ST7 Polyclonal antibody (11945-1-AP)

    WB analysis using 11945-1-AP (same clone as 11945-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11945-1-AP (ST7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11945-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using ST7 Polyclonal antibody (11945-1-AP)

    WB analysis of mouse brain using 11945-1-AP (same clone as 11945-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 11945-1-AP (ST7 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11945-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ST7 Polyclonal antibody (11945-1-AP)

    IHC staining of human liver cancer using 11945-1-AP (same clone as 11945-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11945-1-AP (ST7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11945-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ST7 Polyclonal antibody (11945-1-AP)

    IHC staining of human liver cancer using 11945-1-AP (same clone as 11945-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11945-1-AP (ST7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11945-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using ST7 Polyclonal antibody (11945-1-AP)

    IHC staining of human pancreas using 11945-1-AP (same clone as 11945-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas using 11945-1-AP (ST7 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11945-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using ST7 Polyclonal antibody (11945-1-AP)

    IHC staining of human pancreas using 11945-1-AP (same clone as 11945-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas using 11945-1-AP (ST7 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11945-1-PBS in a different storage buffer formulation.

ST7 Polyclonal antibody, PBS Only

ST7 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 11945-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11945-1-PBS targets ST7 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag2547

Product name: Recombinant human ST7 protein

Source: e coli.-derived, T-GST

Tag: GST

Domain: 101-481 aa of BC030954

Sequence: LRPLLGGVDNNSSNNSDSNRQSVSECKVWRNPLNLFRGAEYNRYTWVTGREPLTYYDMNLSAQDHQTFFTCDSDHLRPADAIMQKAWRERNPQARISAAHEALEINECATAYILLAEEEATTIAEAEKLFKQALKAGDGCYRRSQQLQHHGSQYEAQHRRDTNVLVYIKRRLAMCARRLGRTREAVKMMRDLMKEFPLLSMFNIHENLLEALLELQAYADVQAVLAKYDDISLPKSATICYTAALLKARAVSDKFSPEAASRRGLSTAEMNAVEAIHRAVEFNPHVPKYLLEMKSLILPPEHILKRGDSEAIAYAFFHLAHWKRVEGALNLLHCTWEGTFRMIPYPLEKGHLFYPYPICTETADRELLPSFHEVSVYPKKE

相同性解析による交差性が予測される生物種
Full Name suppression of tumorigenicity 7
Calculated molecular weight 63 kDa
Observed molecular weight 63 kDa
GenBank accession numberBC030954
Gene Symbol ST7
Gene ID (NCBI) 7982
RRIDAB_2270936
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NRC1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Human ST7 (suppressor of tumorigenicity 7) gene, also called FAM4A1, HELG or RAY1, maps to a region on chromosome 7 identified as an autism-susceptibility locus. ST7 has been proposed to be a tumor suppressor gene with highest expression in heart, liver and pancreas. The product of this gene is a multi-pass membrane protein belonging to the ST7 family, full-length ST7 cDNA has been cloned and verified in cancer cell lines, recent result suggests that ST7 may mediate tumor suppression through the regulation of the genes involved in maintaining the cellular structure of the cell and involved in oncogenic pathways.

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