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ST6GAL1 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 39.1-2500 pg/mL

Cat no : MP02980-2

Print datasheet

Synonyms

Alpha 2,6-ST 1, B-cell antigen CD75, Beta-galactoside alpha-2,6-sialyltransferase 1, CD75, CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1

Validation Data Gallery

  • Sandwich ELISA standard curve of MP02980-2, Human ST6GAL1 Recombinant Matched Antibody Pair - PBS only. 87412-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg5012. 87412-3-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

    Sandwich ELISA standard curve of MP02980-2

    Sandwich ELISA standard curve of MP02980-2, Human ST6GAL1 Recombinant Matched Antibody Pair - PBS only. 87412-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg5012. 87412-3-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

  • Plasma of six individual healthy human donors was measured. The ST6GAL1 concentration of detected samples was determined to be 1,794.3 pg/mL with a range of 786.9-2,709.1 pg/mL.

    Sandwich ELISA sample test of MP02980-2

    Plasma of six individual healthy human donors was measured. The ST6GAL1 concentration of detected samples was determined to be 1,794.3 pg/mL with a range of 786.9-2,709.1 pg/mL.

  • HeLa were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for human ST6GAL1, and measured 46.0 pg/mL.

    Sandwich ELISA sample test of MP02980-2

    HeLa were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for human ST6GAL1, and measured 46.0 pg/mL.

  • The mean ST6GAL1 concentration was determined to be 23.1 ng/mL in Raji cell extract based on a 1.2 mg/mL extract load.

    Sandwich ELISA sample test of MP02980-2

    The mean ST6GAL1 concentration was determined to be 23.1 ng/mL in Raji cell extract based on a 1.2 mg/mL extract load.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP02980-2 targets ST6GAL1 in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 39.1-2500 pg/mL (Sandwich ELISA);

Capture antibody: ST6GAL1 Recombinant monoclonal antibody, PBS Only (Capture) 87412-4-PBS (251718G3). 100 μg. Concentration 1 mg/ml.

Detection antibody: ST6GAL1 Recombinant monoclonal antibody, PBS Only (Detector) 87412-3-PBS (251718G5). 100 μg. Concentration 1 mg/ml.

Alternative ST6GAL1 matched antibody pairs: MP02980-1, MP51249-1, MP51249-2, MP51249-3

Matched pair protein standard: Eg5012

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 87412-4-PBS
Tested Reactivity human
Immunogen ST6GAL1 fusion protein Eg5012
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ST6 beta-galactosamide alpha-2,6-sialyltranferase 1
Synonyms
GenBank accession numberBC040009
Gene symbol ST6GAL1
UniprotP15907
Gene ID (NCBI) 6480
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 87412-3-PBS
Tested Reactivity human
Immunogen ST6GAL1 fusion protein Eg5012
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ST6 beta-galactosamide alpha-2,6-sialyltranferase 1
Synonyms
GenBank accession numberBC040009
Gene symbol ST6GAL1
UniprotP15907
Gene ID (NCBI) 6480
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
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