Validation Data Gallery
Tested Applications
| Positive WB detected in | 4T1 cells, mouse liver tissue, rat liver tissue |
| Positive IHC detected in | mouse liver tissue, mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | 4T1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87468-1-RR targets ST6GAL1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with mouse, rat samples.
| Tested Reactivity | mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg5251 Product name: Recombinant mouse ST6GAL1 protein Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 27-403 aa of NM_001252505.1 Sequence: KKGSDYEALTLQAKVFQMPKSQEKVAVGPAPQAVFSNSKQDPKEGVQILSYPRVTAKVKPQPSLQVWDKDSTYSKLNPRLLKIWRNYLNMNKYKVSYKGPGPGVKFSVEALRCHLRDHVNVSMIEATDFPFNTTEWEGYLPKENFRTKAGPWHKCAVVSSAGSLKNSQLGREIDNHDAVLRFNGAPTDNFQQDVGTKTTIRLVNSQLVTTEKRFLKDSLYTEGILILWDPSVYHADIPQWYQKPDYNFFETYKSYRRLHPSQPFYILKPQMPWELWDIIQEISPDLIQPNPPSSGMLGIIIMMTLCDQVDIYEFLPSKRKTDVCYYHQKFFDSACTMGAYHPLLFEKNMVKHLNEGTDEDIYLFGKATLSGFRNNRC 相同性解析による交差性が予測される生物種 |
| Full Name | beta galactoside alpha 2,6 sialyltransferase 1 |
| Calculated molecular weight | 47 kDa |
| Observed molecular weight | 39-42 kDa and 49-50 kDa |
| GenBank accession number | NM_001252505.1 |
| Gene Symbol | St6gal1 |
| Gene ID (NCBI) | 20440 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q64685 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ST6GAL1 is a sialyltransferase mediating the glycosylation of proteins and lipids to form functionally important glycoproteins and glycolipids in the Golgi compartment. It is principally expressed in liver, placenta, and skeletal muscle (PMID: 15049997, PMID: 23358684). Two molecular forms of ST6GAL1 are generally observed, a larger 49-50 kDa and a smaller 39-42 kDa form, representing its full-length and the smaller soluble catalytic domain, respectively (PMID: 35676533, PMID: 35661210).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ST6GAL1 antibody 87468-1-RR | Download protocol |
| IHC protocol for ST6GAL1 antibody 87468-1-RR | Download protocol |
| WB protocol for ST6GAL1 antibody 87468-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |