Validation Data Gallery
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Tested Applications
| Positive WB detected in | A431 cells, SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
30996-1-AP targets ST5 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ST5 fusion protein Ag34032 相同性解析による交差性が予測される生物種 |
| Full Name | suppression of tumorigenicity 5 |
| Calculated molecular weight | 1137 aa, 126 kDa |
| Observed molecular weight | 120-150 kDa |
| GenBank accession number | BC036655 |
| Gene Symbol | ST5 |
| Gene ID (NCBI) | 6764 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P78524 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ST5, also known as DEN2B (DENN domain-containing protein 2B), has three isoforms with molecular weights of 126 kDa, 82 kDa, and 70 kDa, respectively. The mainstream molecular weight is 126kd. ST5 is widely expressed except in peripheral blood lymphocytes. Isoform 1 is expressed in several epithelial cells and fibroblasts (including tumorigenic cells), but not in lymphoid cell lines (at the protein level).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ST5 antibody 30996-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |