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Validation Data Gallery

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  • Western Blot (WB) analysis of human testis tissue using ST5 Polyclonal antibody (12893-1-AP)

    WB analysis of human testis using 12893-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 12893-1-AP (ST5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using ST5 Polyclonal antibody (12893-1-AP)

    WB analysis of SH-SY5Y using 12893-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 12893-1-AP (ST5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ST5 Polyclonal antibody (12893-1-AP)

    WB analysis of HeLa using 12893-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12893-1-AP (ST5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse uterus tissue using ST5 Polyclonal antibody (12893-1-AP)

    WB analysis of mouse uterus using 12893-1-AP

    mouse uterus tissue were subjected to SDS PAGE followed by western blot with 12893-1-AP (ST5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human adrenal gland tissue using ST5 Polyclonal antibody (12893-1-AP)

    WB analysis of human adrenal gland using 12893-1-AP

    human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 12893-1-AP (ST5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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ST5 Polyclonal antibody

ST5 Polyclonal Antibody for WB, ELISA
Cat No. 12893-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

DENN domain-containing protein 2B, DENND2B, HTS1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman testis tissue, SH-SY5Y cells, HeLa cells, human adrenal gland tissue, mouse uterus tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12893-1-AP targets ST5 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ST5 fusion protein Ag3403 相同性解析による交差性が予測される生物種
Full Name suppression of tumorigenicity 5
Calculated molecular weight 1137 aa, 126 kDa
Observed molecular weight 82 kDa, 70 kDa
GenBank accession numberBC036655
Gene Symbol ST5
Gene ID (NCBI) 6764
RRIDAB_10640670
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP78524
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Widely expressed with the exception of peripheral blood lymphocytes. Isoform 1 is expressed in several epithelial and fibroblast (including tumorigenic) but absent in lymphoid cell lines (at protein level).

Protocols

Product Specific Protocols
WB protocol for ST5 antibody 12893-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols