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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ST14 Polyclonal antibody

ST14 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 27176-1-AP

Synonyms

HAI, Matriptase, MT SP1, MTSP 1, MTSP1, Prostamin, PRSS14, Serine protease 14, Serine protease TADG 15, SNC19, ST14, TADG 15, TADG15

Validation Data Gallery

Applications:
  • WB analysis of MCF-7 using 27176-1-AP

    WB analysis of MCF-7 using 27176-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 27176-1-AP (ST14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of MCF-7 using 27176-1-AP

    WB analysis of MCF-7 using 27176-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 27176-1-AP (ST14 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 27176-1-AP

    IHC staining of human colon using 27176-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27176-1-AP (ST14 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human colon using 27176-1-AP

    IHC staining of human colon using 27176-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27176-1-AP (ST14 antibody) at dilution of 1:200 (under 40x lens).

  • IHC staining of human stomach using 27176-1-AP

    IHC staining of human stomach using 27176-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 27176-1-AP (ST14 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human stomach using 27176-1-AP

    IHC staining of human stomach using 27176-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 27176-1-AP (ST14 antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inMCF-7 cells
Positive IHC detected inhuman colon tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 27176-1-AP is ST14 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ST14 fusion protein Ag26007 相同性解析による交差性が予測される生物種
Full Name suppression of tumorigenicity 14 (colon carcinoma)
Calculated molecular weight 46 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC030532
Gene symbol ST14
Gene ID (NCBI) 6768
RRIDAB_2880788
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Suppression of tumorigenicity 14(ST14), is an epithelial-derived, integral membrane serine protease. ST14 forms a complex with the Kunitz-type serine protease inhibitor, HAI-1, and is found to be activated by sphingosine 1-phosphate. ST14 can cleave and activate hepatocyte growth factor/scattering factor, and uPA, which suggest the function of this protease as an epithelial membrane activator for other proteases and latent growth factors.

Protocols

Product Specific Protocols
WB protocol for ST14 antibody 27176-1-APDownload protocol
IHC protocol for ST14 antibody 27176-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC,IF,CoIP

BMC Cancer

ST14 interacts with TMEFF1 and is a predictor of poor prognosis in ovarian cancer

Authors - Xin Nie
View Article