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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using ST14 Polyclonal antibody (27176-1-AP)

    WB analysis of MCF-7 using 27176-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 27176-1-AP (ST14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using ST14 Polyclonal antibody (27176-1-AP)

    WB analysis of MCF-7 using 27176-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 27176-1-AP (ST14 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using ST14 Polyclonal antibody (27176-1-AP)

    IHC staining of human colon using 27176-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27176-1-AP (ST14 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using ST14 Polyclonal antibody (27176-1-AP)

    IHC staining of human colon using 27176-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27176-1-AP (ST14 antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human stomach tissue using ST14 Polyclonal antibody (27176-1-AP)

    IHC staining of human stomach using 27176-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 27176-1-AP (ST14 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human stomach tissue using ST14 Polyclonal antibody (27176-1-AP)

    IHC staining of human stomach using 27176-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 27176-1-AP (ST14 antibody) at dilution of 1:200 (under 40x lens).

ST14 Polyclonal antibody

ST14 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27176-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, CoIP, ELISA

Synonyms

HAI, Matriptase, MT SP1, MTSP 1, MTSP1, Prostamin, PRSS14, Serine protease 14, Serine protease TADG 15, SNC19, ST14, TADG 15, TADG15

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF-7 cells
Positive IHC detected inhuman colon tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27176-1-AP targets ST14 in WB, IF, IHC, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag26007

Product name: Recombinant human ST14 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 301-539 aa of BC030532

Sequence: YNLTFHSSQNVLLITLITNTERRHPGFEATFFQLPRMSSCGGRLRKAQGTFNSPYYPGHYPPNIDCTWNIEVPNNQHVKVRFKFFYLLEPGVPAGTCPKDYVEINGEKYCGERSQFVVTSNSNKITVRFHSDQSYTDTGFLAEYLSYDSSDPCPGQFTCRTGRCIRKELRCDGWADCTDHSDELNCSCDAGHQFTCKNKFCKPLFWVCDSVNDCGDNSDEQGCSCPAQTFRCSNGKCLS

相同性解析による交差性が予測される生物種
Full Name suppression of tumorigenicity 14 (colon carcinoma)
Calculated molecular weight 46 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC030532
Gene Symbol ST14
Gene ID (NCBI) 6768
RRIDAB_2880788
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y5Y6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Suppression of tumorigenicity 14(ST14), is an epithelial-derived, integral membrane serine protease. ST14 forms a complex with the Kunitz-type serine protease inhibitor, HAI-1, and is found to be activated by sphingosine 1-phosphate. ST14 can cleave and activate hepatocyte growth factor/scattering factor, and uPA, which suggest the function of this protease as an epithelial membrane activator for other proteases and latent growth factors.

Protocols

Product Specific Protocols
IHC protocol for ST14 antibody 27176-1-APDownload protocol
WB protocol for ST14 antibody 27176-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC,IF,CoIP

BMC Cancer

ST14 interacts with TMEFF1 and is a predictor of poor prognosis in ovarian cancer

Authors - Xin Nie
View Article
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