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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using SSX4 Polyclonal antibody (15876-1-AP)

    WB analysis of MCF-7 using 15876-1-AP (same clone as 15876-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 15876-1-AP (SSX4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15876-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using SSX4 Polyclonal antibody (15876-1-AP)

    WB analysis of A549 using 15876-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 15876-1-AP (SSX4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using SSX4 Polyclonal antibody (15876-1-AP)

    WB analysis of K-562 using 15876-1-AP (same clone as 15876-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 15876-1-AP (SSX4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15876-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using SSX4 Polyclonal antibody (15876-1-AP)

    IHC staining of human testis using 15876-1-AP (same clone as 15876-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 15876-1-AP (SSX4 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 15876-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using SSX4 Polyclonal antibody (15876-1-AP)

    IHC staining of human testis using 15876-1-AP (same clone as 15876-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 15876-1-AP (SSX4 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 15876-1-PBS in a different storage buffer formulation.

SSX4 Polyclonal antibody, PBS Only

SSX4 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15876-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15876-1-PBS targets SSX4 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag8637

Product name: Recombinant human SSX4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-188 aa of BC005325

Sequence: MNGDDAFARRPRDDAQISEKLRKAFDDIAKYFSKKEWEKMKSSEKIVYVYMKLNYEVMTKLGFKVTLPPFMRSKRAADFHGNDFGNDRNHRNQVERPQMTFGSLQRIFPKIMPKKPAEEENGLKEVPEASGPQNDGKQLCPPGNPSTLEKINKTSGPKRGKHAWTHRLRERKQLVVYEEISDPEEDDE

相同性解析による交差性が予測される生物種
Full Name synovial sarcoma, X breakpoint 4
Calculated molecular weight 188 aa, 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC005325
Gene Symbol SSX4
Gene ID (NCBI) 6759
RRIDAB_2035018
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO60224
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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