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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using SSRP1 Recombinant antibody (84673-5-RR)

    WB analysis of HEK-293 using 84673-5-RR

    WB result of SSRP1 antibody (84673-5-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-SSRP1 transfected HEK-293 cells.

  • Western Blot (WB) analysis of various lysates using SSRP1 Recombinant antibody (84673-5-RR)

    WB analysis using 84673-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84673-5-RR (SSRP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SSRP1 Recombinant monoclonal antibody (84673-5-RR)

    IF Staining of HeLa using 84673-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SSRP1 antibody (84673-5-RR, Clone: 242102H8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using SSRP1 Recombinant antibody (84673-5-RR)

    FC experiment of HeLa using 84673-5-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug SSRP1 Recombinant antibody (84673-5-RR, Clone:242102H8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Flow cytometry (FC) experiment of SH-SY5Y cells using SSRP1 Recombinant antibody (84673-5-RR)

    FC experiment of SH-SY5Y using 84673-5-RR

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug SSRP1 Recombinant antibody (84673-5-RR, Clone:242102H8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO Validated

SSRP1 Recombinant monoclonal antibody

SSRP1 Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 84673-5-RR
Clone No.242102H8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

242102H8, Chromatin-specific transcription elongation factor 80 kDa subunit, Facilitates chromatin transcription complex 80 kDa subunit, Facilitates chromatin transcription complex subunit SSRP1, FACT

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, K-562 cells, Jurkat cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells, SH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84673-5-RR targets SSRP1 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag8318

Product name: Recombinant human SSRP1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 361-709 aa of BC005116

Sequence: YVHKPPVHIRFDEISFVNFARGTTTTRSFDFEIETKQGTQYTFSSIEREEYGKLFDFVNAKKLNIKNRGLKEGMNPSYDEYADSDEDQHDAYLERMKEEGKIREENANDSSDDSGEETDESFNPGEEEEDVAEEFDSNASASSSSNEGDSDRDEKKRKQLKKAKMAKDRKSRKKPVEVKKGKDPNAPKRPMSAYMLWLNASREKIKSDHPGISITDLSKKAGEIWKGMSKEKKEEWDRKAEDARRDYEKAMKEYEGGRGESSKRDKSKKKKKVKVKMEKKSTPSRGSSSKSSSRQLSESFKSKEFVSSDESSSGENKSKKKRRRSEDSEEEELASTPPSSEDSASGSDE

相同性解析による交差性が予測される生物種
Full Name structure specific recognition protein 1
Calculated molecular weight 81 kDa
Observed molecular weight86 kDa
GenBank accession numberBC005116
Gene Symbol SSRP1
Gene ID (NCBI) 6749
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ08945
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
FC protocol for SSRP1 antibody 84673-5-RRDownload protocol
IF protocol for SSRP1 antibody 84673-5-RRDownload protocol
WB protocol for SSRP1 antibody 84673-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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