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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SSRP1 Monoclonal antibody (67313-1-Ig)

    WB analysis using 67313-1-Ig (same clone as 67313-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67313-1-Ig (SSRP1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67313-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using SSRP1 Monoclonal antibody (67313-1-Ig)

    WB analysis using 67313-1-Ig (same clone as 67313-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67313-1-Ig (SSRP1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67313-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-12 cells using SSRP1 Monoclonal antibody (67313-1-Ig)

    WB analysis of PC-12 using 67313-1-Ig (same clone as 67313-1-PBS)

    PC-12 cells were subjected to SDS PAGE followed by western blot with 67313-1-Ig (SSRP1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67313-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of 4T1 cells using SSRP1 Monoclonal antibody (67313-1-Ig)

    WB analysis of 4T1 using 67313-1-Ig (same clone as 67313-1-PBS)

    4T1 cells were subjected to SDS PAGE followed by western blot with 67313-1-Ig (SSRP1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67313-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using SSRP1 Monoclonal antibody (67313-1-Ig)

    IHC staining of human ovary tumor using 67313-1-Ig (same clone as 67313-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67313-1-Ig (SSRP1 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67313-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SSRP1 Monoclonal antibody (67313-1-Ig)

    IHC staining of human breast cancer using 67313-1-Ig (same clone as 67313-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67313-1-Ig (SSRP1 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67313-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SSRP1 Monoclonal antibody (67313-1-Ig)

    IHC staining of human breast cancer using 67313-1-Ig (same clone as 67313-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67313-1-Ig (SSRP1 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67313-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using SSRP1 Monoclonal antibody (67313-1-Ig)

    IHC staining of human ovary tumor using 67313-1-Ig (same clone as 67313-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67313-1-Ig (SSRP1 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67313-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SSRP1 Monoclonal antibody (67313-1-Ig)

    IF Staining of HepG2 using 67313-1-Ig (same clone as 67313-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 67313-1-Ig (SSRP1 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67313-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SSRP1 Monoclonal antibody (67313-1-Ig)

    IF Staining of HepG2 using 67313-1-Ig (same clone as 67313-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SSRP1 antibody (67313-1-Ig, Clone: 3D3H4 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67313-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

SSRP1 Monoclonal antibody, PBS Only

SSRP1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67313-1-PBS
Clone No.3D3H4

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

3D3H4, Chromatin-specific transcription elongation factor 80 kDa subunit, Facilitates chromatin transcription complex 80 kDa subunit, Facilitates chromatin transcription complex subunit SSRP1, FACT

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67313-1-PBS targets SSRP1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag8318

Product name: Recombinant human SSRP1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 361-709 aa of BC005116

Sequence: YVHKPPVHIRFDEISFVNFARGTTTTRSFDFEIETKQGTQYTFSSIEREEYGKLFDFVNAKKLNIKNRGLKEGMNPSYDEYADSDEDQHDAYLERMKEEGKIREENANDSSDDSGEETDESFNPGEEEEDVAEEFDSNASASSSSNEGDSDRDEKKRKQLKKAKMAKDRKSRKKPVEVKKGKDPNAPKRPMSAYMLWLNASREKIKSDHPGISITDLSKKAGEIWKGMSKEKKEEWDRKAEDARRDYEKAMKEYEGGRGESSKRDKSKKKKKVKVKMEKKSTPSRGSSSKSSSRQLSESFKSKEFVSSDESSSGENKSKKKRRRSEDSEEEELASTPPSSEDSASGSDE

相同性解析による交差性が予測される生物種
Full Name structure specific recognition protein 1
Calculated molecular weight 81 kDa
Observed molecular weight 86 kDa
GenBank accession numberBC005116
Gene Symbol SSRP1
Gene ID (NCBI) 6749
RRIDAB_2882573
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ08945
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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